View source for 4v95


==Crystal structure of YAEJ bound to the 70S ribosome==
<StructureSection load='4v95' size='340' side='right'caption='[[4v95]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v95]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4dh9 4dh9], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4dha 4dha], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4dhb 4dhb] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4dhc 4dhc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V95 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v95 OCA], [https://pdbe.org/4v95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v95 RCSB], [https://www.ebi.ac.uk/pdbsum/4v95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v95 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL32_THET8 RL32_THET8] Found on the solvent side of the large subunit.[HAMAP-Rule:MF_00340]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In bacteria, the hybrid transfer-messenger RNA (tmRNA) rescues ribosomes stalled on defective messenger RNAs (mRNAs). However, certain gram-negative bacteria have evolved proteins that are capable of rescuing stalled ribosomes in a tmRNA-independent manner. Here, we report a 3.2 angstrom-resolution crystal structure of the rescue factor YaeJ bound to the Thermus thermophilus 70S ribosome in complex with the initiator tRNA(i)(fMet) and a short mRNA. The structure reveals that the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes by binding in the mRNA entry channel downstream of the A site between the head and shoulder of the 30S subunit. This allows the N-terminal globular domain to sample different conformations, so that its conserved GGQ motif is optimally positioned to catalyze the hydrolysis of peptidyl-tRNA. This structure gives insights into the mechanism of YaeJ function and provides a basis for understanding how it rescues stalled ribosomes.

Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.,Gagnon MG, Seetharaman SV, Bulkley D, Steitz TA Science. 2012 Mar 16;335(6074):1370-2. PMID:22422986<ref>PMID:22422986</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v95" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Bulkley DP]]
[[Category: Gagnon MG]]
[[Category: Seetharaman SV]]
[[Category: Steitz TA]]