View source for 5gw9


==Crystal structure of C163, a backbone circularized G-CSF==
<StructureSection load='5gw9' size='340' side='right'caption='[[5gw9]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5gw9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GW9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gw9 OCA], [https://pdbe.org/5gw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gw9 RCSB], [https://www.ebi.ac.uk/pdbsum/5gw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gw9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSF3_HUMAN CSF3_HUMAN] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
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== Publication Abstract from PubMed ==
Backbone circularization is a powerful approach for enhancing the structural stability of polypeptides. Herein, we present the crystal structure of the circularized variant of the granulocyte colony-stimulating factor (G-CSF) in which the terminal helical region was circularized using a short, two-amino acid connector. The structure revealed that the N- and C-termini were indeed connected by a peptide bond. The local structure of the C-terminal region transited from an alpha helix to 310 helix with a bend close to the N-terminal region, indicating that the structural change offset the insufficient length of the connector. This is the first-ever report of a crystal structure of the backbone of a circularized protein. It will facilitate the development of backbone circularization methodology.

Structural insights into the backbone-circularized granulocyte colony-stimulating factor containing a short connector.,Miyafusa T, Shibuya R, Honda S Biochem Biophys Res Commun. 2018 Apr 16. pii: S0006-291X(18)30808-8. doi:, 10.1016/j.bbrc.2018.04.045. PMID:29634929<ref>PMID:29634929</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5gw9" style="background-color:#fffaf0;"></div>

==See Also==
*[[Colony-stimulating factor 3D structures|Colony-stimulating factor 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Honda S]]
[[Category: Miyafusa T]]