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==The C2 domain of cytosolic phospholipase A2 alpha bound to phosphatidylcholine==
<StructureSection load='6iej' size='340' side='right'caption='[[6iej]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6iej]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IEJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.206&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HXG:1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>HXG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iej OCA], [https://pdbe.org/6iej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iej RCSB], [https://www.ebi.ac.uk/pdbsum/6iej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iej ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA24A_CHICK PA24A_CHICK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ca(2+)-stimulated translocation of cytosolic phospholipase A2alpha (cPLA2alpha) to the Golgi induces arachidonic acid production, the rate-limiting step in pro-inflammatory eicosanoid synthesis. Structural insights into the cPLA2alpha preference for phosphatidylcholine (PC)-enriched membranes have remained elusive. Here, we report cPLA2alpha C2-domain structure (2.2A resolution) containing bound 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) and Ca(2+) ions. Two Ca(2+) are complexed at locations previously reported for lipid-free C2-domain. One of these Ca(2+) along with a third Ca(2+) bridge the C2-domain to the DHPC phosphate group, which also interacts with Asn65. Tyr96 plays a key role in lipid headgroup recognition via cation-pi interaction with the PC trimethylammonium group. Mutagenesis analyses confirm Tyr96 and Asn65 function in PC binding selectivity by C2-domain and regulation of cPLA2alpha activity. The differing DHPC-binding mode of cPLA2alpha C2-domain, compared to phosphatidylserine or phosphatidylinositol 4,5-bisphosphate binding by other C2-domains, expands and deepens knowledge of lipid-binding mechanisms mediated by C2-domains.

Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase A2alpha.,Hirano Y, Gao YG, Stephenson DJ, Vu NT, Malinina L, Simanshu DK, Chalfant CE, Patel DJ, Brown RE Elife. 2019 May 3;8. pii: 44760. doi: 10.7554/eLife.44760. PMID:31050338<ref>PMID:31050338</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6iej" style="background-color:#fffaf0;"></div>

==See Also==
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Brown RE]]
[[Category: Chalfant CE]]
[[Category: Gao YG]]
[[Category: Hirano Y]]
[[Category: Malinina L]]
[[Category: Patel DJ]]
[[Category: Stephenson DJ]]
[[Category: Vu NT]]