View source for 6jkw


==Seleno-methionine PNGM-1 from deep-sea sediment metagenome==
<StructureSection load='6jkw' size='340' side='right'caption='[[6jkw]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jkw]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JKW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jkw OCA], [http://pdbe.org/6jkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jkw RCSB], [http://www.ebi.ac.uk/pdbsum/6jkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jkw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
OBJECTIVES: In order to find antimicrobial resistance gene(s) pre-dating the use of antibiotics through metagenomics, functional screening of a metagenomic library from the deep-seep sediments of Edison Seamount (ca. 10000 years old) was performed. METHODS: Among 60 antimicrobial-resistant clones, a single clone with the highest minimum inhibitory concentration (MIC) for ampicillin was selected. Sequence analysis revealed a new metallo-beta-lactamase (MBL) gene, designated as blaPNGM-1. PNGM-1 retains a zinc ion-binding motif (H116XH118XD120H121, H196 and H263), conserved in subclass B3 MBLs. The catalytic parameters of purified PNGM-1 and the MICs of beta-lactams for Escherichia coli TOP10 transformants harbouring the blaPNGM-1 gene were assessed. RESULTS: Antimicrobial susceptibility testing indicated reduced susceptibility to penicillins, narrow- and extended-spectrum cephalosporins, and carbapenems in E. coli TOP10 transformants harbouring the blaPNGM-1 gene. In addition, kinetic analyses revealed that PNGM-1 hydrolysed almost all beta-lactams. CONCLUSIONS: The PNGM-1 enzyme is the first case of a subclass B3 MBL derived from a functional metagenomic library of a deep-sea sediment that pre-dates the antibiotic era.

PNGM-1, a novel subclass B3 metallo-beta-lactamase from a deep-sea sediment metagenome.,Park KS, Kim TY, Kim JH, Lee JH, Jeon JH, Karim AM, Malik SK, Lee SH J Glob Antimicrob Resist. 2018 Sep;14:302-305. doi: 10.1016/j.jgar.2018.05.021., Epub 2018 May 26. PMID:29842976<ref>PMID:29842976</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jkw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Hong, M K]]
[[Category: Jeon, J H]]
[[Category: Kang, L W]]
[[Category: Lee, J H]]
[[Category: Lee, S H]]
[[Category: Park, K S]]
[[Category: Park, Y S]]
[[Category: Antibiotic resistance]]
[[Category: Mbl]]
[[Category: Metagenome]]
[[Category: Psi-biology]]
[[Category: Structural genomic]]
[[Category: Transferase]]
[[Category: Zinc binding motif]]