View source for 6lcj


==TtGalA, alpha-galactosidase from Thermus thermopilus in apo form==
<StructureSection load='6lcj' size='340' side='right'caption='[[6lcj]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lcj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LCJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lcj OCA], [https://pdbe.org/6lcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lcj RCSB], [https://www.ebi.ac.uk/pdbsum/6lcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lcj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q53W51_THET8 Q53W51_THET8] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
alpha-Galactosidase catalyzes the hydrolysis of a terminal alpha-galactose residue in galacto-oligosaccharides and has potential in various industrial applications and food processing. We determined the crystal structures of alpha-galactosidase from the thermophilic microorganism Thermus thermophilus (TtGalA) and its complexes with pNPGal and stachyose. The monomer folds into an N-terminal domain, a catalytic (beta/alpha)8 barrel domain, and a C-terminal domain. The domain organization is similar to that of the other family of 36 alpha-galactosidases, but TtGalA presents a cagelike hexamer. Structural analysis shows that oligomerization may be a key factor for the thermal adaption of TtGalA. The structure of TtGalA complexed with stachyose reveals only the existence of one -1 subsite and one +1 subsite in the active site. Structural comparison of the stachyose-bound complexes of TtGalA and GsAgaA, a tetrameric enzyme with four subsites, suggests evolutionary divergence of substrate specificity within the GH36 family of alpha-galactosidases. To the best of our knowledge, the crystal structure of TtGalA is the first report of a quaternary structure as a hexameric assembly in the alpha-galactosidase family.

Crystal Structure of alpha-Galactosidase from Thermus thermophilus: Insight into Hexamer Assembly and Substrate Specificity.,Chen SC, Wu SP, Chang YY, Hwang TS, Lee TH, Hsu CH J Agric Food Chem. 2020 Jun 3;68(22):6161-6169. doi: 10.1021/acs.jafc.0c00871., Epub 2020 May 20. PMID:32390413<ref>PMID:32390413</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lcj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Chen SC]]
[[Category: Hsu CH]]