View source for 6te4


==Structural insights into Pseudomonas aeruginosa Type six secretion system exported effector 8: Tse8 in complex with a peptide==
<StructureSection load='6te4' size='340' side='right'caption='[[6te4]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6te4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TE4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6te4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6te4 OCA], [https://pdbe.org/6te4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6te4 RCSB], [https://www.ebi.ac.uk/pdbsum/6te4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6te4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HWL8_PSEAE Q9HWL8_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recent reports indicate that the Type six secretion system exported effector 8 (Tse8) is a cytoactive effector secreted by the Type VI secretion system (T6SS) of the human pathogen Pseudomonas aeruginosa. The T6SS is a nanomachine that assembles inside of the bacteria and injects effectors/toxins into target cells, providing a fitness advantage over competing bacteria and facilitating host colonisation. Here we present the first crystal structure of Tse8 revealing that it conserves the architecture of the catalytic triad Lys84-transSer162-Ser186 that characterises members of the Amidase Signature superfamily. Furthermore, using binding affinity experiments, we show that the interaction of phenylmethylsulfonyl fluoride (PMSF) to Tse8 is dependent on the putative catalytic residue Ser186, providing support for its nucleophilic reactivity. This work thus demonstrates that Tse8 belongs to the Amidase Signature (AS) superfamily. Furthermore, it highlights Tse8 similarity to two family members: the Stenotrophomonas maltophilia Peptide Amidase and the Glutamyl-tRNA(Gln) amidotransferase subunit A from Staphylococcus aureus.

Structural insights into Pseudomonas aeruginosaType six secretion system exported effector 8.,Gonzalez-Magana A, Sainz-Polo MA, Pretre G, Capuni R, Lucas M, Altuna J, Montanchez I, Fucini P, Albesa-Jove D J Struct Biol. 2020 Oct 20;212(3):107651. doi: 10.1016/j.jsb.2020.107651. PMID:33096229<ref>PMID:33096229</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6te4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Albesa-Jove D]]
[[Category: Altuna J]]
[[Category: Capuni R]]
[[Category: Fucini P]]
[[Category: Lucas M]]
[[Category: Montanchez I]]
[[Category: Sainz-Polo MA]]