View source for 6z0w


==Crystal structure of the cytoplasmic domain of FlhB from Shewanella putrefaciens==
<StructureSection load='6z0w' size='340' side='right'caption='[[6z0w]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6z0w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_putrefaciens_CN-32 Shewanella putrefaciens CN-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z0W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z0w OCA], [https://pdbe.org/6z0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z0w RCSB], [https://www.ebi.ac.uk/pdbsum/6z0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z0w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4Y8K1_SHEPC A4Y8K1_SHEPC] Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.[ARBA:ARBA00025078][RuleBase:RU364091]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flagella are bacterial organelles of locomotion. Their biogenesis is highly coordinated in time and space and relies on a specialized flagellar type III secretion system (fT3SS) required for the assembly of the extracellular hook, rod, and filament parts of this complex motor device. The fT3SS protein FlhB switches secretion substrate specificity once the growing hook reaches its determined length. Here we present the crystal structure of the cytoplasmic domain of the transmembrane protein FlhB. The structure visualizes a so-far unseen proline-rich region (PRR) at the very C-terminus of the protein. Strains lacking the PRR show a decrease in flagellation as determined by hook- and filament staining, indicating a role of the PRR during assembly of the hook and filament structures. Phylogenetic analysis shows that the PRR is a primary feature of FlhB proteins of flagellated beta- and gamma-proteobacteria. Taken together, our study adds another layer of complexity and organismic diversity to the process of flagella biogenesis.

A Proline-Rich Element in the Type III Secretion Protein FlhB Contributes to Flagellar Biogenesis in the Beta- and Gamma-Proteobacteria.,Hook JC, Blagotinsek V, Pane-Farre J, Mrusek D, Altegoer F, Dornes A, Schwan M, Schier L, Thormann KM, Bange G Front Microbiol. 2020 Dec 15;11:564161. doi: 10.3389/fmicb.2020.564161., eCollection 2020. PMID:33384667<ref>PMID:33384667</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6z0w" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Shewanella putrefaciens CN-32]]
[[Category: Altegoer F]]
[[Category: Bange G]]