View source for 7bmh


==Crystal structure of a light-driven proton pump LR (Mac) from Leptosphaeria maculans==
<StructureSection load='7bmh' size='340' side='right'caption='[[7bmh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bmh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptosphaeria_maculans Leptosphaeria maculans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BMH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bmh OCA], [https://pdbe.org/7bmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bmh RCSB], [https://www.ebi.ac.uk/pdbsum/7bmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bmh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HGT7_LEPMC Q9HGT7_LEPMC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early evolution of life on earth. Whereas archaeal and bacterial proton pumps are among the best structurally characterized proteins, rhodopsins from unicellular eukaryotes have not been well characterized. To fill this gap in the current understanding of the proton pumps and to gain insight into the evolution of rhodopsins using a structure-based approach, we performed a structural and functional analysis of the light-driven proton pump LR (Mac) from the pathogenic fungus Leptosphaeria maculans. The first high-resolution structure of fungi rhodopsin and its functional properties reveal the striking similarity of its membrane part to archaeal but not to bacterial rhodopsins. We show that an unusually long N-terminal region stabilizes the protein through direct interaction with its extracellular loop (ECL2). We compare to our knowledge all available structures and sequences of outward light-driven proton pumps and show that eukaryotic and archaeal proton pumps, most likely, share a common ancestor.

Structure-based insights into evolution of rhodopsins.,Zabelskii D, Dmitrieva N, Volkov O, Shevchenko V, Kovalev K, Balandin T, Soloviov D, Astashkin R, Zinovev E, Alekseev A, Round E, Polovinkin V, Chizhov I, Rogachev A, Okhrimenko I, Borshchevskiy V, Chupin V, Buldt G, Yutin N, Bamberg E, Koonin E, Gordeliy V Commun Biol. 2021 Jun 30;4(1):821. doi: 10.1038/s42003-021-02326-4. PMID:34193947<ref>PMID:34193947</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bmh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Leptosphaeria maculans]]
[[Category: Astashkin R]]
[[Category: Dmitrieva N]]
[[Category: Gordeliy V]]
[[Category: Kovalev K]]
[[Category: Shevchenko V]]
[[Category: Volkov O]]
[[Category: Zabelskii D]]
[[Category: Zinovev E]]