View source for 7ctn


==Structure of the 328-692 fragment of FlhA (E351A/D356A)==
<StructureSection load='7ctn' size='340' side='right'caption='[[7ctn]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ctn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CTN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ctn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ctn OCA], [https://pdbe.org/7ctn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ctn RCSB], [https://www.ebi.ac.uk/pdbsum/7ctn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ctn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FLHA_SALTY FLHA_SALTY] Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The flagellar protein export apparatus switches substrate specificity from hook-type to filament-type upon hook assembly completion, thereby initiating filament assembly at the hook tip. The C-terminal cytoplasmic domain of FlhA (FlhA(C)) serves as a docking platform for flagellar chaperones in complex with their cognate filament-type substrates. Interactions of the flexible linker of FlhA (FlhA(L)) with its nearest FlhA(C) subunit in the FlhA(C) ring is required for the substrate specificity switching. To address how FlhA(L) brings the order to flagellar assembly, we analyzed the flhA(E351A/W354A/D356A) DeltaflgM mutant and found that this triple mutation in FlhA(L) increased the secretion level of hook protein by 5-fold, thereby increasing hook length. The crystal structure of FlhA(C)(E351A/D356A) showed that FlhA(L) bound to the chaperone-binding site of its neighboring subunit. We propose that the interaction of FlhA(L) with the chaperon-binding site of FlhA(C) suppresses filament-type protein export and facilitates hook-type protein export during hook assembly.

The FlhA linker mediates flagellar protein export switching during flagellar assembly.,Inoue Y, Kinoshita M, Kida M, Takekawa N, Namba K, Imada K, Minamino T Commun Biol. 2021 May 31;4(1):646. doi: 10.1038/s42003-021-02177-z. PMID:34059784<ref>PMID:34059784</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ctn" style="background-color:#fffaf0;"></div>

==See Also==
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
[[Category: Imada K]]
[[Category: Inoue Y]]
[[Category: Kida M]]
[[Category: Kinoshita M]]
[[Category: Minamino T]]
[[Category: Takekawa N]]