View source for 7efo


==LptB2FG-LPS from Klebsiella pneumoniae in nanodiscs==
<StructureSection load='7efo' size='340' side='right'caption='[[7efo]], [[Resolution|resolution]] 3.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7efo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EFO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EFO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JSG:(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-5-[(2~{S},3~{S},4~{R},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{R},5~{S},6~{R})-6-[(1~{S})-2-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-1-oxidanyl-ethyl]-3,4-bis(oxidanyl)-5-phosphonooxy-oxan-2-yl]oxy-3-oxidanyl-5-phosphonooxy-oxan-2-yl]oxy-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic+acid'>JSG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7efo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7efo OCA], [https://pdbe.org/7efo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7efo RCSB], [https://www.ebi.ac.uk/pdbsum/7efo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7efo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2X3II39_KLEPN A0A2X3II39_KLEPN] Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system.[ARBA:ARBA00024818]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven lipopolysaccharide transport proteins (LptA-G). LptB(2)FG, an type VI ATP-binding cassette (ABC) transporter, forms a stable complex with LptC, extracts LPS from the IM and powers LPS transport to the OM. Here we report the cryo-EM structures of LptB(2)FG and LptB(2)FGC from Klebsiella pneumoniae in complex with LPS. The KpLptB(2)FG-LPS structure provides detailed interactions between LPS and the transporter, while the KpLptB(2)FGC-LPS structure may represent an intermediate state that the transmembrane helix of LptC has not been fully inserted into the transmembrane domains of LptB(2)FG.

Cryo-EM structures of LptB(2)FG and LptB(2)FGC from Klebsiella pneumoniae in complex with lipopolysaccharide.,Luo Q, Shi H, Xu X Biochem Biophys Res Commun. 2021 Sep 24;571:20-25. doi: , 10.1016/j.bbrc.2021.07.049. Epub 2021 Jul 21. PMID:34303191<ref>PMID:34303191</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7efo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsiella pneumoniae subsp. pneumoniae]]
[[Category: Large Structures]]
[[Category: Luo Q]]
[[Category: Shi H]]