View source for 7ekd


==Crystal structure of gibberellin 3-oxidase 2 (GA3ox2) in rice==
<StructureSection load='7ekd' size='340' side='right'caption='[[7ekd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ekd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EKD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.899&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=J5X:Gibberellin+A9'>J5X</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ekd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ekd OCA], [https://pdbe.org/7ekd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ekd RCSB], [https://www.ebi.ac.uk/pdbsum/7ekd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ekd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G3OX2_ORYSJ G3OX2_ORYSJ] Catalyzes the 3-beta-hydroxylation of the inactive gibberellin precursors, leading to the formation of bioactive gibberellins. In vitro, converts the precursors GA20, GA5, GA44 and GA9 to the corresponding 3-beta-hydroxylated active products GA1, GA3, GA38 and GA4, respectively. Involved in the production of bioactive GA for vegetative growth and development (PubMed:11438692). Controls the elongation of the vegetative shoot and plant height by the regulation of active gibberellin levels (Ref.7).<ref>PMID:11438692</ref> [EMBL:BAB17075.1]
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== Publication Abstract from PubMed ==
Proper anther and pollen development are important for plant reproduction. The plant hormone gibberellin is important for anther development in rice, but its gametophytic functions remain largely unknown. Here, we report the functional and evolutionary analyses of rice gibberellin 3-oxidase 1 (OsGA3ox1), a gibberellin synthetic enzyme specifically expressed in the late developmental stages of anthers. Enzymatic and X-ray crystallography analyses reveal that OsGA3ox1 has a higher GA7 synthesis ratio than OsGA3ox2. In addition, we generate an osga3ox1 knockout mutant by genome editing and demonstrate the bioactive gibberellic acid synthesis by the OsGA3ox1 action during starch accumulation in pollen via invertase regulation. Furthermore, we analyze the evolution of Oryza GA3ox1s and reveal that their enzyme activity and gene expression have evolved in a way that is characteristic of the Oryza genus and contribute to their male reproduction ability.

Evolutionary alterations in gene expression and enzymatic activities of gibberellin 3-oxidase 1 in Oryza.,Kawai K, Takehara S, Kashio T, Morii M, Sugihara A, Yoshimura H, Ito A, Hattori M, Toda Y, Kojima M, Takebayashi Y, Furuumi H, Nonomura KI, Mikami B, Akagi T, Sakakibara H, Kitano H, Matsuoka M, Ueguchi-Tanaka M Commun Biol. 2022 Jan 19;5(1):67. doi: 10.1038/s42003-022-03008-5. PMID:35046494<ref>PMID:35046494</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ekd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Kawai K]]
[[Category: Mikami B]]
[[Category: Takehara S]]
[[Category: Ueguchi-Tanaka M]]