View source for 7emy


==Pyochelin synthetase, a dimeric nonribosomal peptide synthetase elongation module==
<StructureSection load='7emy' size='340' side='right'caption='[[7emy]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7emy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EMY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.97&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7emy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7emy OCA], [https://pdbe.org/7emy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7emy RCSB], [https://www.ebi.ac.uk/pdbsum/7emy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7emy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PCHE_PSEAE PCHE_PSEAE] Involved in the biosynthesis of the siderophore pyochelin (PubMed:9846750, PubMed:11208777). Accepts salicylate activated by PchD at the first peptidyl carrier domain (ArCP), and activates and fixes one molecule of cysteine at the second peptidyl carrier domain (PCP1) via a thioester linkage to the phosphopanthetheine moiety (By similarity). Then catalyzes the condensation reaction between the salicylate bound to the first site and the cysteine bound to the second site, and the cyclization of the cysteine to form the salicyl-thiazolinyl-S-PCP1 intermediate at the second site (By similarity). When this intermediate is released by the action of a thioesterase, it produces the antifungal antibiotic dihydroaeruginoic acid (Dha or hydroxyphenyl-thiazolinyl-carboxylate) (PubMed:11208777).[UniProtKB:A0A0H2ZGB9]<ref>PMID:11208777</ref> <ref>PMID:9846750</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Wang JL]]
[[Category: Wang ZJ]]