View source for 7fiw


==Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidAwMel(ST) and CidBND1-ND2 from wPip(Pel)==
<StructureSection load='7fiw' size='340' side='right'caption='[[7fiw]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7fiw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolbachia_endosymbiont_of_Culex_pipiens Wolbachia endosymbiont of Culex pipiens], [https://en.wikipedia.org/wiki/Wolbachia_endosymbiont_of_Culex_quinquefasciatus_Pel Wolbachia endosymbiont of Culex quinquefasciatus Pel] and [https://en.wikipedia.org/wiki/Wolbachia_endosymbiont_of_Drosophila_melanogaster Wolbachia endosymbiont of Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FIW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fiw OCA], [https://pdbe.org/7fiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fiw RCSB], [https://www.ebi.ac.uk/pdbsum/7fiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fiw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B3CP63_WOLPP B3CP63_WOLPP] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA(wMel) and CidB(wMel). Substituting CidA(wMel) interface residues with those from CidA(wPip) (from strain wPip) enables the mutant protein to bind CidB(wPip) and rescue CidB(wPip)-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA(wPip) and CidB(wPip) proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains.

Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.,Wang H, Xiao Y, Chen X, Zhang M, Sun G, Wang F, Wang L, Zhang H, Zhang X, Yang X, Li W, Wei Y, Yao D, Zhang B, Li J, Cui W, Wang F, Chen C, Shen W, Su D, Bai F, Huang J, Ye S, Zhang L, Ji X, Wang W, Wang Z, Hochstrasser M, Yang H Nat Commun. 2022 Mar 25;13(1):1608. doi: 10.1038/s41467-022-29273-w. PMID:35338130<ref>PMID:35338130</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7fiw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Wolbachia endosymbiont of Culex pipiens]]
[[Category: Wolbachia endosymbiont of Culex quinquefasciatus Pel]]
[[Category: Wolbachia endosymbiont of Drosophila melanogaster]]
[[Category: Chen X]]
[[Category: Ji XY]]
[[Category: Wang W]]
[[Category: Xiao YJ]]
[[Category: Yang HT]]