View source for 7qla


==Structure of the Rab GEF complex Mon1-Ccz1==
<StructureSection load='7qla' size='340' side='right'caption='[[7qla]], [[Resolution|resolution]] 3.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qla]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QLA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qla OCA], [https://pdbe.org/7qla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qla RCSB], [https://www.ebi.ac.uk/pdbsum/7qla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qla ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G0SGS3_CHATD G0SGS3_CHATD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Activation of the GTPase Rab7/Ypt7 by its cognate guanine nucleotide exchange factor (GEF) Mon1-Ccz1 marks organelles such as endosomes and autophagosomes for fusion with lysosomes/vacuoles and degradation of their content. Here, we present a high-resolution cryogenic electron microscopy structure of the Mon1-Ccz1 complex that reveals its architecture in atomic detail. Mon1 and Ccz1 are arranged side by side in a pseudo-twofold symmetrical heterodimer. The three Longin domains of each Mon1 and Ccz1 are triangularly arranged, providing a strong scaffold for the catalytic center of the GEF. At the opposite side of the Ypt7-binding site, a positively charged and relatively flat patch stretches the Longin domains 2/3 of Mon1 and functions as a phosphatidylinositol phosphate-binding site, explaining how the GEF is targeted to membranes. Our work provides molecular insight into the mechanisms of endosomal Rab activation and serves as a blueprint for understanding the function of members of the Tri Longin domain Rab-GEF family.

Structure of the Mon1-Ccz1 complex reveals molecular basis of membrane binding for Rab7 activation.,Klink BU, Herrmann E, Antoni C, Langemeyer L, Kiontke S, Gatsogiannis C, Ungermann C, Raunser S, Kummel D Proc Natl Acad Sci U S A. 2022 Feb 8;119(6):e2121494119. doi: , 10.1073/pnas.2121494119. PMID:35105815<ref>PMID:35105815</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qla" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Chaetomium thermophilum]]
[[Category: Large Structures]]
[[Category: Antoni C]]
[[Category: Gatsogiannis C]]
[[Category: Herrmann E]]
[[Category: Kiontke S]]
[[Category: Klink BU]]
[[Category: Kuemmel D]]
[[Category: Langemeyer L]]
[[Category: Raunser S]]
[[Category: Ungermann C]]