View source for 7spu


==In situ cryo-EM structure of bacteriophage Sf6 gp3:gp7:gp5 complex in conformation 1 at 3.73A resolution==
<StructureSection load='7spu' size='340' side='right'caption='[[7spu]], [[Resolution|resolution]] 3.73&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7spu]] is a 54 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_virus_Sf6 Shigella virus Sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.73&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spu OCA], [https://pdbe.org/7spu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spu RCSB], [https://www.ebi.ac.uk/pdbsum/7spu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q716G8_BPSFV Q716G8_BPSFV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sf6 is a bacterial virus that infects the human pathogen Shigella flexneri. Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6 degrees with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae.

High-resolution cryo-EM structure of the Shigella virus Sf6 genome delivery tail machine.,Li F, Hou CD, Yang R, Whitehead R 3rd, Teschke CM, Cingolani G Sci Adv. 2022 Dec 9;8(49):eadc9641. doi: 10.1126/sciadv.adc9641. Epub 2022 Dec 7. PMID:36475795<ref>PMID:36475795</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7spu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Shigella virus Sf6]]
[[Category: Cingolani G]]
[[Category: Hou C]]
[[Category: Li F]]
[[Category: Yang R]]