View source for 7vwv


==The crystal structure of African swine fever virus I73R==
<StructureSection load='7vwv' size='340' side='right'caption='[[7vwv]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vwv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VWV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vwv OCA], [https://pdbe.org/7vwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vwv RCSB], [https://www.ebi.ac.uk/pdbsum/7vwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vwv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2X0RU36_ASF A0A2X0RU36_ASF] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
African swine fever virus (ASFV) is a large, double-stranded DNA virus that causes a fatal disease in pigs, posing a threat to the global pig industry. Whereas some ASFV proteins have been found to play important roles in ASFV-host interaction, the functional roles of many proteins are still largely unknown. In this study, we identified I73R, an early viral gene in the replication cycle of ASFV, as a key virulence factor. Our findings demonstrate that pI73R suppresses the host innate immune response by broadly inhibiting the synthesis of host proteins, including antiviral proteins. Crystallization and structural characterization results suggest that pI73R is a nucleic-acid-binding protein containing a Zalpha domain. It localizes in the nucleus and inhibits host protein synthesis by suppressing the nuclear export of cellular messenger RNA (mRNAs). While pI73R promotes viral replication, the deletion of the gene showed that it is a nonessential gene for virus replication. In vivo safety and immunogenicity evaluation results demonstrate that the deletion mutant ASFV-GZDeltaI73R is completely nonpathogenic and provides effective protection to pigs against wild-type ASFV. These results reveal I73R as a virulence-related gene critical for ASFV pathogenesis and suggest that it is a potential target for virus attenuation. Accordingly, the deletion mutant ASFV-GZDeltaI73R can be a potent live-attenuated vaccine candidate.

African swine fever virus I73R is a critical virulence-related gene: A potential target for attenuation.,Liu Y, Shen Z, Xie Z, Song Y, Li Y, Liang R, Gong L, Di D, Liu J, Liu J, Chen Z, Yu W, Lv L, Zhong Q, Liao X, Tian C, Wang R, Song Q, Wang H, Peng G, Chen H Proc Natl Acad Sci U S A. 2023 Apr 11;120(15):e2210808120. doi: , 10.1073/pnas.2210808120. Epub 2023 Apr 6. PMID:37023125<ref>PMID:37023125</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7vwv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: African swine fever virus]]
[[Category: Large Structures]]
[[Category: Liang R]]
[[Category: Shen Z]]