View source for 7wkj


==A COVID-19 T-cell response detection method based on a newly identified human CD8+ T cell epitope from SARS-CoV-2-Hubei Province, 2021.==
<StructureSection load='7wkj' size='340' side='right'caption='[[7wkj]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wkj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WKJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wkj OCA], [https://pdbe.org/7wkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wkj RCSB], [https://www.ebi.ac.uk/pdbsum/7wkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wkj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A6M6CC39_HUMAN A0A6M6CC39_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
INTRODUCTION: Similar to antibody detection, severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-specific T-cell response evaluation is also pivotal among the coronavirus disease 2019 (COVID-19) convalescents and the vaccinated populations. Nucleocapsid (N) protein is one of the main structural proteins of SARS-CoV-2 and can trigger T-cell responses in humans. METHODS: An overlapping peptide pool covering the full length of the N protein was designed, peptides with positive T-cell activating potency in COVID-19 convalescents were screened, and CD8(+) T cell epitopes were further identified. The epitope was used to detect the SARS-CoV-2-specific CD8(+) T cell responses in COVID-19 convalescents based in intracellular cytokine staining and tetramer staining in flow cytometry. RESULTS: A human leukocyte antigen A (HLA-A)*1101-restricted CD8(+) T cell epitope, which could stimulate the production of IFN-gamma via peripheral blood mononuclear cells (PBMCs) of the convalescents was defined, and the tetramer generated with this epitope could detect SARS-CoV-2-specific T cells in the PBMCs of the convalescents. The structural investigation eliminated that the epitope was a typical HLA-A*1101-restricted T-cell epitope which was conserved among all the sarbecoviruses. DISCUSSION: The newly identified SARS-CoV-2-derived T-cell epitope was helpful to detect the cellular immunity against different sarbecoviruses including SARS-CoV and SARS-CoV-2. This study provided an evaluation method and also a peptide candidate for the research and development of T-cell based vaccine for the virus.

A COVID-19 T-Cell Response Detection Method Based on a Newly Identified Human CD8(+) T Cell Epitope from SARS-CoV-2 - Hubei Province, China, 2021.,Zhang J, Lu D, Li M, Liu M, Yao S, Zhan J, Liu WJ, Gao GF China CDC Wkly. 2022 Feb 4;4(5):83-87. doi: 10.46234/ccdcw2021.258. PMID:35186375<ref>PMID:35186375</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7wkj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Gao GF]]
[[Category: Li M]]
[[Category: Liu J]]
[[Category: Liu MS]]
[[Category: Lu D]]
[[Category: Yao SJ]]
[[Category: Zhan JB]]
[[Category: Zhang J]]