View source for 8cmn


==18mer DNA mimic Foldamer with an aliphatic linker in complex with Sac7d wild protein==
<StructureSection load='8cmn' size='340' side='right'caption='[[8cmn]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8cmn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CMN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOA:GLYCOLIC+ACID'>GOA</scene>, <scene name='pdbligand=V4F:8-(aminomethyl)-4-(phosphonomethoxy)quinoline-2-carboxylic+acid'>V4F</scene>, <scene name='pdbligand=V53:8-azanyl-4-(phosphonomethoxy)quinoline-2-carboxylic+acid'>V53</scene>, <scene name='pdbligand=V5F:(2~{R})-2-(2-azanylphenoxy)propanoic+acid'>V5F</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cmn OCA], [https://pdbe.org/8cmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cmn RCSB], [https://www.ebi.ac.uk/pdbsum/8cmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cmn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length. The interaction is diastereoselective and takes place at the DNA binding site. Crystallography revealed that the DNA mimic foldamers have a binding mode of their own and that they can bind to Sac7d without being kinked.

DNA Mimic Foldamer Recognition of a Chromosomal Protein.,Deepak D, Wu J, Corvaglia V, Allmendinger L, Scheckenbach M, Tinnefeld P, Huc I Angew Chem Int Ed Engl. 2024 Dec 23:e202422958. doi: 10.1002/anie.202422958. PMID:39714421<ref>PMID:39714421</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8cmn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfolobus acidocaldarius DSM 639]]
[[Category: Synthetic construct]]
[[Category: Corvaglia V]]
[[Category: Deepak D]]
[[Category: Huc I]]
[[Category: Wu J]]