View source for 8fr5


==Crystal structure of the Human Smacovirus 1 Rep domain==
<StructureSection load='8fr5' size='340' side='right'caption='[[8fr5]], [[Resolution|resolution]] 1.33&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8fr5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_smacovirus_1 Human smacovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FR5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fr5 OCA], [https://pdbe.org/8fr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fr5 RCSB], [https://www.ebi.ac.uk/pdbsum/8fr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fr5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0B5CMT4_9VIRU A0A0B5CMT4_9VIRU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 A resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

The crystal structure of the human smacovirus 1 Rep domain.,Limon LK, Shi K, Dao A, Rugloski J, Tompkins KJ, Aihara H, Gordon WR, Evans RL 3rd Acta Crystallogr F Struct Biol Commun. 2023 Dec 1;79(Pt 12):295-300. doi: , 10.1107/S2053230X23009536. Epub 2023 Dec 5. PMID:38051309<ref>PMID:38051309</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8fr5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human smacovirus 1]]
[[Category: Large Structures]]
[[Category: Aihara H]]
[[Category: Dao A]]
[[Category: Evans IIII RL]]
[[Category: Gordon WR]]
[[Category: Limon LK]]
[[Category: Rugloski J]]
[[Category: Shi K]]
[[Category: Tompkins KJ]]