View source for 8ikw


==A complex structure of PGIP-PG==
<StructureSection load='8ikw' size='340' side='right'caption='[[8ikw]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ikw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_phyllophilum Fusarium phyllophilum] and [https://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IKW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ikw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ikw OCA], [https://pdbe.org/8ikw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ikw RCSB], [https://www.ebi.ac.uk/pdbsum/8ikw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ikw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Polygalacturonase-inhibiting proteins (PGIPs) interact with pathogen-derived polygalacturonases to inhibit their virulence-associated plant cell wall-degrading activity but stimulate immunity-inducing oligogalacturonide production. Here we show that interaction between Phaseolus vulgaris PGIP2 (PvPGIP2) and Fusarium phyllophilum polygalacturonase (FpPG) enhances substrate binding, resulting in inhibition of the enzyme activity of FpPG. This interaction promotes FpPG-catalyzed production of long-chain immunoactive oligogalacturonides, while diminishing immunosuppressive short oligogalacturonides. PvPGIP2 binding creates a substrate binding site on PvPGIP2-FpPG, forming a new polygalacturonase with boosted substrate binding activity and altered substrate preference. Structure-based engineering converts a putative PGIP that initially lacks FpPG-binding activity into an effective FpPG-interacting protein. These findings unveil a mechanism for plants to transform pathogen virulence activity into a defense trigger and provide proof of principle for engineering PGIPs with broader specificity.

A plant mechanism of hijacking pathogen virulence factors to trigger innate immunity.,Xiao Y, Sun G, Yu Q, Gao T, Zhu Q, Wang R, Huang S, Han Z, Cervone F, Yin H, Qi T, Wang Y, Chai J Science. 2024 Feb 16;383(6684):732-739. doi: 10.1126/science.adj9529. Epub 2024 , Feb 15. PMID:38359129<ref>PMID:38359129</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ikw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Fusarium phyllophilum]]
[[Category: Large Structures]]
[[Category: Phaseolus vulgaris]]
[[Category: Chai J]]
[[Category: Xiao Y]]