View source for 8ipf


==Crystal structure of an ankyrin protein that can specifically recognize a parallel G-quadruplex==
<StructureSection load='8ipf' size='340' side='right'caption='[[8ipf]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ipf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IPF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ipf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ipf OCA], [https://pdbe.org/8ipf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ipf RCSB], [https://www.ebi.ac.uk/pdbsum/8ipf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ipf ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
G-Quadruplex (G4) structures formed by guanine-rich DNA and RNA sequences are implicated in various biological processes. Understanding the mechanisms by which proteins recognize G4 structures is crucial for elucidating their functional roles. Here we present the X-ray crystal structure of an ankyrin protein bound to a parallel G4 structure. Our findings reveal a new specific recognition mode in which a bundle of alpha-helices and loops of the ankyrin form a flat surface to stack on the G-tetrad core. The protein employs a combination of hydrogen bonds and hydrophobic contacts to interact with the G4, and electrostatic interaction is used to enhance the binding affinity. This binding mechanism provides valuable insights into understanding G4 recognition by proteins.

Structural Basis for Parallel G-Quadruplex Recognition by an Ankyrin Protein.,Ngo KH, Liew CW, Heddi B, Phan AT J Am Chem Soc. 2024 May 22;146(20):13709-13713. doi: 10.1021/jacs.4c01971. Epub , 2024 May 13. PMID:38738955<ref>PMID:38738955</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ipf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Heddi B]]
[[Category: Liew CW]]
[[Category: Ngo KH]]
[[Category: Phan AT]]