View source for 8skw


==MicroED structure of d(CGCGCG)2 Z-DNA==
<StructureSection load='8skw' size='340' side='right'caption='[[8skw]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8skw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SKW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8skw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8skw OCA], [https://pdbe.org/8skw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8skw RCSB], [https://www.ebi.ac.uk/pdbsum/8skw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8skw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)(2) DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 A resolution structure of the d(CGCGCG)(2) DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

Structure determination of a DNA crystal by MicroED.,Haymaker A, Bardin AA, Gonen T, Martynowycz MW, Nannenga BL Structure. 2023 Jul 24:S0969-2126(23)00248-4. doi: 10.1016/j.str.2023.07.005. PMID:37541248<ref>PMID:37541248</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8skw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Bardin AA]]
[[Category: Haymaker A]]
[[Category: Martynowycz MW]]
[[Category: Nannenga BL]]