View source for 8vxq


==Cryo-EM structure of phage DEV ejection proteins gp72:gp73==
<StructureSection load='8vxq' size='340' side='right'caption='[[8vxq]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8vxq]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_vB_PaeP_DEV Pseudomonas phage vB_PaeP_DEV]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VXQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vxq OCA], [https://pdbe.org/8vxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vxq RCSB], [https://www.ebi.ac.uk/pdbsum/8vxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vxq ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo-electron microscopy localized reconstruction, biochemical methods, and genetic knockouts. We built de novo structures of all capsid factors and tail components involved in host attachment. We demonstrate that DEV long tail fibers are essential for infection of Pseudomonas aeruginosa but dispensable for infecting mutants with a truncated lipopolysaccharide devoid of the O-antigen. We determine that DEV vRNAP is part of a three-gene operon conserved in 191 Schitoviridae genomes. We propose these three proteins are ejected into the host to form a genome ejection motor spanning the cell envelope. We posit that the design principles of the DEV ejection apparatus are conserved in all Schitoviridae.

Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor.,Lokareddy RK, Hou CD, Forti F, Iglesias SM, Li F, Pavlenok M, Horner DS, Niederweis M, Briani F, Cingolani G Nat Commun. 2024 Oct 1;15(1):8482. doi: 10.1038/s41467-024-52752-1. PMID:39353939<ref>PMID:39353939</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas phage vB_PaeP_DEV]]
[[Category: Cingolani G]]
[[Category: Iglesias SM]]