View source for 8yqh


==Crystal structure of Cry3Aa-PEI at 3.1 A==
<StructureSection load='8yqh' size='340' side='right'caption='[[8yqh]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8yqh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YQH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.11&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8yqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8yqh OCA], [https://pdbe.org/8yqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8yqh RCSB], [https://www.ebi.ac.uk/pdbsum/8yqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8yqh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9S6N9_BACTU Q9S6N9_BACTU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
NAD(H)-dependent enzymes play a crucial role in the biosynthesis of pharmaceuticals and fine chemicals, but the limited recyclability of the NAD(H) cofactor hinders its more general application. Here, we report the generation of mechano-responsive PEI-modified Cry3Aa protein crystals and their use for NADH recycling over multiple reaction cycles. For demonstration of its practical utility, a complementary Cry3Aa protein particle containing genetically encoded and co-immobilized formate dehydrogenase for NADH regeneration and leucine dehydrogenase for catalyzing the NADH-dependent l-tert-leucine (l-tert-Leu) biosynthesis has been produced. When combined with the PEI-modified Cry3Aa crystal, the resultant reaction system could be used for the efficient biosynthesis of l-tert-Leu for up to 21 days with a 10.5-fold improvement in the NADH turnover number.

Mechanoresponsive Protein Crystals for NADH Recycling in Multicycle Enzyme Reactions.,Yekta R, Xiong X, Li J, Heater BS, Lee MM, Chan MK J Am Chem Soc. 2024 Jul 17;146(28):18817-18822. doi: 10.1021/jacs.4c04725. Epub , 2024 Jul 5. PMID:38968608<ref>PMID:38968608</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8yqh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Bacillus thuringiensis]]
[[Category: Large Structures]]
[[Category: Chan MK]]
[[Category: Li J]]