View source for 8zym


==Complex structure of 60 Fab bound to DS2 prefusion F trimer==
<StructureSection load='8zym' size='340' side='right'caption='[[8zym]], [[Resolution|resolution]] 3.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8zym]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus], [https://en.wikipedia.org/wiki/Respiratory_syncytial_virus_A2 Respiratory syncytial virus A2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZYM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.23&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zym OCA], [https://pdbe.org/8zym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zym RCSB], [https://www.ebi.ac.uk/pdbsum/8zym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zym ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C3UPB8_9MONO C3UPB8_9MONO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DS-Cav1, SC-TM, and DS2 are distinct designer pre-fusion F proteins (pre-F) of respiratory syncytial virus (RSV) developed for vaccines. However, their immunogenicity has not been directly compared. In this study, we generated three recombinant vaccines using the chimpanzee adenovirus vector AdC68 to express DS-Cav1, SC-TM, and DS2. All three vaccines elicited robust serum binding and neutralizing antibodies following intramuscular priming and boosting. DS2 induced the strongest antibody responses, followed by SC-TM and DS-Cav1. DS2 also provided strong protection against live RSV challenge. Monoclonal antibodies (mAbs) isolated from long-lived antibody-secreting cells (ASCs) in the bone marrow six months post-immunization with AdC68-DS2 predominantly targeted site O as well as site II. One neutralizing antibody against site II, mAb60, conferred strong protection against live RSV infection in mice. These findings highlight the strong ability of the DS2 design in eliciting long-lived antibody responses and guide the development of next-generation RSV vaccines.

DS2 designer pre-fusion F vaccine induces strong and protective antibody response against RSV infection.,Yang Y, Wang R, Guo F, Zhao T, Lei Y, Yang Q, Zeng Y, Yang Z, Ajavavarakula T, Tan R, Li M, Dong H, Niu M, Bao K, Geng H, Lv Q, Zhang Q, Shi X, Liu P, Ge J, Wang X, Zhang L NPJ Vaccines. 2024 Dec 31;9(1):258. doi: 10.1038/s41541-024-01059-9. PMID:39741146<ref>PMID:39741146</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8zym" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Respiratory syncytial virus A2]]
[[Category: Synthetic construct]]
[[Category: Ge J]]
[[Category: Guo F]]
[[Category: Wang X]]