View source for 9i8v


==Cryo-EM structure of the Danio rerio tRNA ligase complex==
<StructureSection load='9i8v' size='340' side='right'caption='[[9i8v]], [[Resolution|resolution]] 3.33&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9i8v]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9I8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9I8V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.33&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9i8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9i8v OCA], [https://pdbe.org/9i8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9i8v RCSB], [https://www.ebi.ac.uk/pdbsum/9i8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9i8v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F1R2L8_DANRE F1R2L8_DANRE] RNA helicase.[RuleBase:RU365068]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In archaea and metazoa, tRNA exons are ligated by the RNA ligases RtcB and RTCB, respectively. The metazoan RTCB forms a stable complex with four additional subunits, DDX1, FAM98B, CGI99 and ASHWIN. The role and assembly of these four components remain elusive. Furthermore, we lack structural information of how RNA substrates are recognized by 3'-5' tRNA ligases. Here, we use thiol-based chemical cross-linking to confirm the involvement of specific residues of RtcB in RNA binding and we present a cryo-electron microscopy structure of the purified five-subunit D. rerio tRNA ligase complex. The structure implies that the DDX1 helicase module is mobile and can modulate the activity of RTCB. Taken together, the presented results enhance our mechanistic understanding of RNA binding by 3'-5' tRNA splicing ligases and architecture of the metazoan tRNA ligase complex.

Structural and biochemical characterization of the 3'-5' tRNA splicing ligases.,Chamera S, Zajko W, Czarnocki-Cieciura M, Jaciuk M, Koziej L, Nowak J, Wycisk K, Sroka M, Chramiec-Glabik A, Smietanski M, Golebiowski F, Warminski M, Jemielity J, Glatt S, Nowotny M J Biol Chem. 2025 Apr 10:108506. doi: 10.1016/j.jbc.2025.108506. PMID:40220997<ref>PMID:40220997</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9i8v" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Danio rerio]]
[[Category: Large Structures]]
[[Category: Chamera S]]
[[Category: Chramiec-Glabik A]]
[[Category: Czarnocki-Cieciura M]]
[[Category: Glatt S]]
[[Category: Golebiowski F]]
[[Category: Jaciuk M]]
[[Category: Jemielity J]]
[[Category: Koziej L]]
[[Category: Nowak J]]
[[Category: Nowotny M]]
[[Category: Smietanski M]]
[[Category: Sroka M]]
[[Category: Warminski M]]
[[Category: Wycisk K]]
[[Category: Zajko W]]