View source for 9qzk


==SadB of Pseudomonas aeruginosa==
<StructureSection load='9qzk' size='340' side='right'caption='[[9qzk]], [[Resolution|resolution]] 3.16&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9qzk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9QZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9QZK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.16&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9qzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9qzk OCA], [https://pdbe.org/9qzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9qzk RCSB], [https://www.ebi.ac.uk/pdbsum/9qzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9qzk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HTL2_PSEAE Q9HTL2_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The ability of bacteria to commit to surface colonization and biofilm formation is a highly regulated process. In this study, we characterized the activity and structure of SadB, initially identified as a key regulator in the transition from reversible to irreversible surface attachment. Our results show that SadB acts as an adaptor protein that tightly regulates the master regulator AmrZ at the post-translational level. SadB directly binds to the C-terminal domain of AmrZ, leading to its rapid degradation, primarily by the Lon protease. Structural analysis suggests that SadB does not directly interact with small molecules upon signal transduction, differing from previous findings in Pseudomonas fluorescens. Instead, the SadB structure supports its role in mediating protein-protein interactions, establishing it as a major checkpoint for biofilm commitment.

SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa.,Ben-David Y, Sporny M, Brochin Y, Piscon B, Roth S, Zander I, Nisani M, Shoshani S, Yaron O, Karako-Lampert S, Lebenthal-Loinger I, Danielli A, Opatowsky Y, Banin E NPJ Biofilms Microbiomes. 2025 May 13;11(1):77. doi: 10.1038/s41522-025-00710-0. PMID:40360526<ref>PMID:40360526</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9qzk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Opatowsky Y]]
[[Category: Sporny M]]