4y1p: Difference between revisions
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==Crystal structure of 3-isopropylmalate dehydrogenase (Saci_0600) from Sulfolobus acidocaldarius complex with 3-isopropylmalate and Mg2+== | |||
<StructureSection load='4y1p' size='340' side='right'caption='[[4y1p]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4y1p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y1P FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPM:3-ISOPROPYLMALIC+ACID'>IPM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1p OCA], [https://pdbe.org/4y1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y1p RCSB], [https://www.ebi.ac.uk/pdbsum/4y1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1p ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q4JB37_SULAC Q4JB37_SULAC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sulfolobus acidocaldarius, a hyperthermoacidophilic archaeon, possesses two beta-decarboxylating dehydrogenase genes, saci_0600 and saci_2375, in its genome, which suggests that it uses these enzymes for three similar reactions in lysine biosynthesis through 2-aminoadipate, leucine biosynthesis, and the tricarboxylic acid cycle. To elucidate their roles, these two genes were expressed in Escherichia coli in the present study and their gene products were characterized. Saci_0600 recognized 3-isopropylmalate as a substrate, but exhibited slight and no activity for homoisocitrate and isocitrate, respectively. Saci_2375 exhibited distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. These results suggest that Saci_0600 is a 3-isopropylmalate dehydrogenase for leucine biosynthesis and Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase. The crystal structure of Saci_0600 was determined as a closed-form complex that binds 3-isopropylmalate and Mg2+, thereby revealing the structural basis for the extreme thermostability and novel-type recognition of the 3-isopropyl moiety of the substrate. | |||
Characterization of two beta-decarboxylating dehydrogenases from Sulfolobus acidocaldarius.,Takahashi K, Nakanishi F, Tomita T, Akiyama N, Lassak K, Albers SV, Kuzuyama T, Nishiyama M Extremophiles. 2016 Nov;20(6):843-853. Epub 2016 Sep 2. PMID:27590116<ref>PMID:27590116</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4y1p" style="background-color:#fffaf0;"></div> | ||
[[Category: Nishiyama | |||
[[Category: Takahashi | ==See Also== | ||
[[Category: | *[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sulfolobus acidocaldarius DSM 639]] | |||
[[Category: Kuzuyama T]] | |||
[[Category: Nishiyama M]] | |||
[[Category: Takahashi K]] | |||
[[Category: Tomita T]] | |||
Latest revision as of 15:33, 8 November 2023
Crystal structure of 3-isopropylmalate dehydrogenase (Saci_0600) from Sulfolobus acidocaldarius complex with 3-isopropylmalate and Mg2+
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