2ey4: Difference between revisions

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[[Image:2ey4.gif|left|200px]]


{{Structure
==Crystal Structure of a Cbf5-Nop10-Gar1 Complex==
|PDB= 2ey4 |SIZE=350|CAPTION= <scene name='initialview01'>2ey4</scene>, resolution 2.11&Aring;
<StructureSection load='2ey4' size='340' side='right'caption='[[2ey4]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[2ey4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] and [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EY4 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
|GENE= truB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02572 PseudoU_synth_hDyskerin], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08068 DKCLD], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam01472 PUA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK04270 PRK04270], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2260 COG2260], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG3277 GAR1]</span>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ey4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ey4 OCA], [https://pdbe.org/2ey4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ey4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ey4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ey4 ProSAT]</span></td></tr>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ey4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ey4 OCA], [http://www.ebi.ac.uk/pdbsum/2ey4 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2ey4 RCSB]</span>
</table>
}}
== Function ==
[https://www.uniprot.org/uniprot/Q8U029_PYRFU Q8U029_PYRFU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/2ey4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ey4 ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of a Cbf5-Nop10-Gar1 Complex'''
==See Also==
 
*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
 
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
==Overview==
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure.
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2EY4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] and [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EY4 OCA].
 
==Reference==
Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita., Rashid R, Liang B, Baker DL, Youssef OA, He Y, Phipps K, Terns RM, Terns MP, Li H, Mol Cell. 2006 Jan 20;21(2):249-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16427014 16427014]
[[Category: Protein complex]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus dsm 3638]]
[[Category: Pyrococcus furiosus DSM 3638]]
[[Category: Li, H.]]
[[Category: Li H]]
[[Category: Liang, B.]]
[[Category: Liang B]]
[[Category: Rashid, R.]]
[[Category: Rashid R]]
[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: secsg]]
[[Category: southeast collaboratory for structural genomic]]
[[Category: structural genomic]]
[[Category: trimeric complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:23:24 2008''

Latest revision as of 09:20, 14 February 2024

Crystal Structure of a Cbf5-Nop10-Gar1 Complex

2ey4, resolution 2.11Å

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