4reb: Difference between revisions

Latest revision as of 12:51, 1 March 2024

Structural Insights into 5' Flap DNA Unwinding and Incision by the Human FAN1 Dimer

Structural highlights

4reb is a 5 chain structure with sequence from Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 4.2Å
Ligands:	SM
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

FAN1_HUMAN Karyomegalic interstitial nephritis. The disease is caused by mutations affecting the gene represented in this entry.

Function

FAN1_HUMAN Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.^[1] ^[2] ^[3] ^[4]

References

↑ MacKay C, Declais AC, Lundin C, Agostinho A, Deans AJ, MacArtney TJ, Hofmann K, Gartner A, West SC, Helleday T, Lilley DM, Rouse J. Identification of KIAA1018/FAN1, a DNA repair nuclease recruited to DNA damage by monoubiquitinated FANCD2. Cell. 2010 Jul 9;142(1):65-76. doi: 10.1016/j.cell.2010.06.021. PMID:20603015 doi:https://dx.doi.org/10.1016/j.cell.2010.06.021
↑ Kratz K, Schopf B, Kaden S, Sendoel A, Eberhard R, Lademann C, Cannavo E, Sartori AA, Hengartner MO, Jiricny J. Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents. Cell. 2010 Jul 9;142(1):77-88. doi: 10.1016/j.cell.2010.06.022. PMID:20603016 doi:https://dx.doi.org/10.1016/j.cell.2010.06.022
↑ Smogorzewska A, Desetty R, Saito TT, Schlabach M, Lach FP, Sowa ME, Clark AB, Kunkel TA, Harper JW, Colaiacovo MP, Elledge SJ. A genetic screen identifies FAN1, a Fanconi anemia-associated nuclease necessary for DNA interstrand crosslink repair. Mol Cell. 2010 Jul 9;39(1):36-47. doi: 10.1016/j.molcel.2010.06.023. PMID:20603073 doi:https://dx.doi.org/10.1016/j.molcel.2010.06.023
↑ Liu T, Ghosal G, Yuan J, Chen J, Huang J. FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair. Science. 2010 Aug 6;329(5992):693-6. doi: 10.1126/science.1192656. Epub 2010 Jul , 29. PMID:20671156 doi:https://dx.doi.org/10.1126/science.1192656

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OCA

[1] MacKay C, Declais AC, Lundin C, Agostinho A, Deans AJ, MacArtney TJ, Hofmann K, Gartner A, West SC, Helleday T, Lilley DM, Rouse J. Identification of KIAA1018/FAN1, a DNA repair nuclease recruited to DNA damage by monoubiquitinated FANCD2. Cell. 2010 Jul 9;142(1):65-76. doi: 10.1016/j.cell.2010.06.021. PMID:20603015 doi:https://dx.doi.org/10.1016/j.cell.2010.06.021

[2] Kratz K, Schopf B, Kaden S, Sendoel A, Eberhard R, Lademann C, Cannavo E, Sartori AA, Hengartner MO, Jiricny J. Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents. Cell. 2010 Jul 9;142(1):77-88. doi: 10.1016/j.cell.2010.06.022. PMID:20603016 doi:https://dx.doi.org/10.1016/j.cell.2010.06.022

[3] Smogorzewska A, Desetty R, Saito TT, Schlabach M, Lach FP, Sowa ME, Clark AB, Kunkel TA, Harper JW, Colaiacovo MP, Elledge SJ. A genetic screen identifies FAN1, a Fanconi anemia-associated nuclease necessary for DNA interstrand crosslink repair. Mol Cell. 2010 Jul 9;39(1):36-47. doi: 10.1016/j.molcel.2010.06.023. PMID:20603073 doi:https://dx.doi.org/10.1016/j.molcel.2010.06.023

[4] Liu T, Ghosal G, Yuan J, Chen J, Huang J. FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair. Science. 2010 Aug 6;329(5992):693-6. doi: 10.1126/science.1192656. Epub 2010 Jul , 29. PMID:20671156 doi:https://dx.doi.org/10.1126/science.1192656

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[2]

[3]

[4]

@@ Line 1: / Line 1: @@
 ==Structural Insights into 5' Flap DNA Unwinding and Incision by the Human FAN1 Dimer==
-<StructureSection load='4reb' size='340' side='right' caption='[[4reb]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
+<StructureSection load='4reb' size='340' side='right'caption='[[4reb]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4reb]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4REB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4REB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4reb]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4REB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4REB FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rea|4rea]], [[4rec|4rec]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4reb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4reb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4reb RCSB], [http://www.ebi.ac.uk/pdbsum/4reb PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4reb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4reb OCA], [https://pdbe.org/4reb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4reb RCSB], [https://www.ebi.ac.uk/pdbsum/4reb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4reb ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/FAN1_HUMAN FAN1_HUMAN]] Karyomegalic interstitial nephritis. The disease is caused by mutations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/FAN1_HUMAN FAN1_HUMAN] Karyomegalic interstitial nephritis. The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/FAN1_HUMAN FAN1_HUMAN]] Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.<ref>PMID:20603015</ref> <ref>PMID:20603016</ref> <ref>PMID:20603073</ref> <ref>PMID:20671156</ref>
+[https://www.uniprot.org/uniprot/FAN1_HUMAN FAN1_HUMAN] Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.<ref>PMID:20603015</ref> <ref>PMID:20603016</ref> <ref>PMID:20603073</ref> <ref>PMID:20671156</ref>
+==See Also==
+*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Longerich, S]]
+[[Category: Homo sapiens]]
-[[Category: Sung, P]]
+[[Category: Large Structures]]
-[[Category: Xiong, Y]]
+[[Category: Synthetic construct]]
-[[Category: Xue, X]]
+[[Category: Longerich S]]
-[[Category: Zhao, Q]]
+[[Category: Sung P]]
-[[Category: Fancid2]]
+[[Category: Xiong Y]]
-[[Category: Hjc]]
+[[Category: Xue X]]
-[[Category: Hydrolase-dna complex]]
+[[Category: Zhao Q]]
-[[Category: Nucleus]]
-[[Category: Sap]]
-[[Category: Structure specific nuclease]]
-[[Category: Tpr]]

4reb: Difference between revisions

Latest revision as of 12:51, 1 March 2024

Structural Insights into 5' Flap DNA Unwinding and Incision by the Human FAN1 Dimer

Structural highlights

Disease

Function

See Also

References

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