9avs: Difference between revisions

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<table><tr><td colspan='2'>[[9avs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9AVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9AVS FirstGlance]. <br>
<table><tr><td colspan='2'>[[9avs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9AVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9AVS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.53&#8491;</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.53&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9avs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9avs OCA], [https://pdbe.org/9avs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9avs RCSB], [https://www.ebi.ac.uk/pdbsum/9avs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9avs ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9avs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9avs OCA], [https://pdbe.org/9avs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9avs RCSB], [https://www.ebi.ac.uk/pdbsum/9avs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9avs ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
== Function ==
[https://www.uniprot.org/uniprot/AGAL_HUMAN AGAL_HUMAN]  
[https://www.uniprot.org/uniprot/AGAL_HUMAN AGAL_HUMAN]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sphingolipid activator protein B (saposin B; SapB) is an essential activator of globotriaosylceramide (Gb3) catabolism by alpha-galactosidase A. However, the manner by which SapB stimulates alpha-galactosidase A activity remains unknown. To uncover the molecular mechanism of SapB presenting Gb3 to alpha-galactosidase A, we subjected the fluorescent substrate globotriaosylceramide-nitrobenzoxidazole (Gb3-NBD) to a series of biochemical and structural assays involving SapB. First, we showed that SapB stably binds Gb3-NBD using a fluorescence equilibrium binding assay, isolates Gb3-NBD from micelles, and facilitates alpha-galactosidase A cleavage of Gb3-NBD in vitro. Second, we crystallized SapB in the presence of Gb3-NBD and validated the ligand-bound assembly. Third, we captured transient interactions between SapB and alpha-galactosidase A by chemical cross-linking. Finally, we determined the crystal structure of SapB bound to alpha-galactosidase A. These findings establish general principles for molecular recognition in saposin:hydrolase complexes and highlight the utility of NBD reporter lipids in saposin biochemistry and structural biology.
Human Saposin B Ligand Binding and Presentation to alpha-Galactosidase A.,Sawyer TK, Aral E, Staros JV, Bobst CE, Garman SC bioRxiv [Preprint]. 2024 Apr 4:2024.04.04.584535. doi: 10.1101/2024.04.04.584535. PMID:38617236<ref>PMID:38617236</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9avs" style="background-color:#fffaf0;"></div>
== References ==
== References ==
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