Intersectin

spinqualitylabelsall models Human intersectin 1 short isoform 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (deepskyblue, pink, light pink, orange) (PDB code 6gbu) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Intersectin (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses. The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains. ITSN 2 is also involved in clathrin-mediated endocytosis and is very similar to ITSN 1. ITSN-1 has two isoforms as a result of differential splicing: ITSN-1 short form is 140kD and expressed in all tissues ITSN-1 long form is 200kD and expressed in neutrons, eye, heart, blood cells and kidney. ITSN-2 short form contains 2 EH domains, a coiled-coil domain and 5 SH3 domains ITSN-2 long formcontains an extra carboxylate domain. Relevance ITSN 1 is expressed in neuroblastoma tumors and tumor cell lines and is necessary for their tumorigenic properties. ITSN 2 might be a potential biomarker for discrimination of severe acute pancreatitis to improve the prognosis of patients. Overexpression of ITSN 1 is implicated for human neurodegenerative diseases such as Down's syndrome and Alzheimer's disease. Structural highlights The 3D structure of the complex between the SH3 domains of ITSN 1 and FCHSD2 shows extensive contact surface composed mainly of hydrophobic interactions.

3D structures of intersectin

Updated on 05-October-2025

{"openlevels":0}

• Intersectin 1 short isoform
  • 3fia - hITSN1 EH domain 1-111
    
  • 2khn - hITSN1 EH domain - NMR
    
  • 2kgr - hITSN1 residues 210-312 - NMR
    
  • 6h5t - hITSN1 1st SH3 domain 741-835
    
  • 4iim - hITSN1 2nd SH3 domain + peptide
    
  • 6gbu - hITSN1 4th SH3 domain 1055-1133 + FCHSD2
    
• Intersectin 1 long isoform
  • 5hzj - hITSN1 DBL+PH domains 1229-1580
    
  • 5hzi - hITSN1 DBL+PH domains (mutant)
    
  • 1ki1, 5hzk - hITSN1 DBL+PH domains + Cdc42
    
  • 3qbv - hITSN1 DBL+PH domains (mutant) + Cdc42
    
  • 3jv3 - mITSN1 5th SH3+DH domains 1151-1431
    
• Intersectin 2 short isoform
  • 1uff - hITSN2 1st SH3 domain 761-841 - NMR
    
  • 1j3t - hITSN2 2nd SH3 domain 897-957 - NMR
    
  • 4iio - hITSN2 2nd SH3 domain + peptide
    
  • 1uhf - hITSN2 3rd SH3 domain 982-1037 - NMR
    
  • 1ue9 - hITSN2 4th SH3 domain 1055-1121 - NMR
    
  • 1udl - hITSN2 5th SH3 domain 1102-1186 - NMR
    
• Intersectin 2 long isoform
  • 3gf9 - hITSN2 RhoGEF domain 1102-1378
    
  • 3jzy - hITSN2 C2 domain 1173-1664
    

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky