6e4v

The Crystal Structure of FhuE from E. coli in complex with its substrate Coprogen

spinqualitylabelsall models 6e4v, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6e4v is a 1 chain structure with sequence from Escherichia coli BW25113. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: BOG, HWS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FHUE_ECOLI Required for the uptake of Fe(3+) via coprogen, ferrioxamine B, and rhodotorulic acid. Publication Abstract from PubMed In order to survive in mixed microbial communities, some species of fungi secrete coprogens, siderophores that facilitate capture of the scarce nutrient iron. The TonB-dependent transporter FhuE is integrated in the outer membrane of Gram-negative bacteria and has been reported to scavenge these fungally produced coprogens. In this work, an Escherichia coli strain was engineered that is dependent solely on FhuE for its access to siderophore-sequestered iron. Using this tool, it is shown that while FhuE is highly active in the import of coprogens, it has some level of promiscuity, acting as a low-affinity transporter for related siderophores. The crystal structure of FhuE in complex with coprogen was determined, providing a structural basis to explain this selective promiscuity. The structural data, in combination with functional analysis, presented in this work show that FhuE has evolved to specifically engage with planar siderophores. A potential evolutionary driver, and a critical consequence of this selectivity, is that it allows FhuE to exclude antibiotics that mimic nonplanar hydroxamate siderophores: these toxic molecules could otherwise cross the outer membrane barrier through a Trojan horse mechanism. Determination of the molecular basis for coprogen import by Gram-negative bacteria.,Grinter R, Lithgow T IUCrJ. 2019 Apr 5;6(Pt 3):401-411. doi: 10.1107/S2052252519002926. eCollection, 2019 May 1. PMID:31098021 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ferric hydroxamate uptake receptor

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