Gyrase: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
David Canner (talk | contribs)
7,699 edits
No edit summary
Michal Harel (talk | contribs)
32,849 edits
No edit summary
 
(51 intermediate revisions by 4 users not shown)
Line 1: Line 1:
[[Image:3L6V.jpg|left|350px]]
<StructureSection load='' size='350' side='right' caption='Gyrase subunit A N-terminal (cyan) and subunit B C-terminal (magenta) complex with DNA, inhibitor, sulfate and Mn+2 ion (purple) (PDB entry [[4plb]])' scene='41/410321/Cv/1'>
==Function==


<applet load='3l6v.pdb' size='350' frame='true' align='right' scene="Gyrase/Gyrase_starting_scene/1" caption= 'Crystal Structure of the Xanthomonas campestris Gyrase A C-terminal Domain, [[3l6v]]' />
'''Gyrase (Gyr)''' is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA.  The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA<ref>PMID:1657531</ref>  Gyr consists of 2 subunits: GyrA and GyrB.  '''Reverse gyrase''' (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil. <ref>PMID:15673717</ref> See also [[Isomerases]].


'''3D Structure of Gyrase''' <br />
==Relevance==
The image at the left is a Crystal Structure of the Gyrase A C-terminal Domain([[3l6v]])


==Gyrase==
GyrA inhibitor [[Ciprofloxacin]] is used as antibiotic drug.  Fluoroquinolones are Gyr inhibitors used in treatment of multi drug-resistant tuberculosis<ref>PMID:15047530</ref>


Gyrase (Gyr) is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA. The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA.  Gyr consists of 2 subunits: GyrA and GyrB.  Reverse gyrase (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil.<br /><br /><br />
== Structural highlights ==
<scene name='41/410321/Cv/5'>Gyrase subunit A N-terminal and subunit B C-terminal complex with DNA and inhibitor</scene>. A <scene name='41/410321/Cv/6'>potential drug interacts with both DNA strands and in the hydrophobic pocket between subunits A and B</scene><ref>PMID:24900889</ref>. Water molecules shown as red spheres.


==3D Structure of Gyrase==
==3D Structure of Gyrase==
[[Gyrase 3D Structures]]


===Gyrase Subunit A===
</StructureSection>


[[3l6v]] – GyrA C-terminal – Xanthomonas campestris <br />
==Additional Resources==
[[2wl2]] – EcGyrA N-terminal+simocylinone – Escherichia coli<br />
For additional information, see: [[Bacterial Infections]]
[[1ajb]] - EcGyrA N-terminal+novobiocin<br />
<br />
[[1zi0]], [[1ab4]] - EcGyrA C-terminal<br />
[[1x75]] – EcGyrA14+CcdB<br />
[[3ilw]] - MtGyrA N-terminal – Mycobacterium tuberculosis<br />
[[1suu]] - GyrA C-terminal – Borrelia burgdorferi<br />


===Gyrase Subunit B===
==References==
<References />


[3g75]], [[3g7b]], [[3g7e]] – GyrB+thiazole inhibitor – Staphylococcus aureus<br />
[[Category:Topic Page]]
[[2cjt]] - MtGyrB C-terminal<br />
[[3cwv]] – GyrB truncated – Myxococcus xanthus<br />
[[1kzn]], [[1ei1]] - EcGyrB N-terminal+clorobiocin<br />
[[1kij]] – GyrB domain+novobiocin – Thermus thermophilus<br />
 
===Reverse Gyrase===
 
[[1gku]] – AfTop-RG – Archaeoglobus fulgidus<br />
[[1gl9]] - AfTop-RG+ADPNP<br />
Retrieved from "https://proteopedia.org/Gyrase"