2rrt: Difference between revisions
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New page: '''Unreleased structure''' The entry 2rrt is ON HOLD Authors: Hirota, h. Description: Solution structure of Magnesium-bound form of calmodulin C-domain E104D/E140D mutant |
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==Solution structure of Magnesium-bound form of calmodulin C-domain E104D/E140D mutant== | |||
<StructureSection load='2rrt' size='340' side='right'caption='[[2rrt]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2rrt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2eqc 2eqc]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RRT FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrt OCA], [https://pdbe.org/2rrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rrt RCSB], [https://www.ebi.ac.uk/pdbsum/2rrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrt ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Calmodulin (CaM) is a Ca(2+)-binding protein that functions as a ubiquitous Ca(2+)-signaling molecule, through conformational changes from the "closed" apo conformation to the "open" Ca(2+)-bound conformation. Mg(2+) also binds to CaM and stabilizes its folded structure, but the NMR signals are broadened by slow conformational fluctuations. Using the E104D/E140D mutant, designed to decrease the signal broadening in the presence of Mg(2+) with minimal perturbations of the overall structure, the solution structure of the Mg(2+)-bound form of the CaM C-terminal domain was determined by multidimensional NMR spectroscopy. The Mg(2+)-induced conformational change mainly occurred in EF hand IV, while EF-hand III retained the apo structure. The helix G and helix H sides of the binding sequence undergo conformational changes needed for the Mg(2+) coordination, and thus the helices tilt slightly. The aromatic rings on helix H move to form a new cluster of aromatic rings in the hydrophobic core. Although helix G tilts slightly to the open orientation, the closed conformation is maintained. The fact that the Mg(2+)-induced conformational changes in EF-hand IV and the hydrophobic core are also seen upon Ca(2+) binding suggests that the Ca(2+)-induced conformational changes can be divided into two categories, those specific to Ca(2+) and those common to Ca(2+) and Mg(2+). | |||
Solution structure and fluctuation of the Mg(2+)-bound form of calmodulin C-terminal domain.,Ohashi W, Hirota H, Yamazaki T Protein Sci. 2011 Apr;20(4):690-701. doi: 10.1002/pro.598. PMID:21312310<ref>PMID:21312310</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2rrt" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Calmodulin 3D structures|Calmodulin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Xenopus laevis]] | |||
[[Category: Hirota H]] | |||
[[Category: Ohashi W]] | |||
[[Category: Yamazaki T]] | |||
Latest revision as of 19:16, 29 May 2024
Solution structure of Magnesium-bound form of calmodulin C-domain E104D/E140D mutant
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