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New page: left|200px<br /><applet load="2f5t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2f5t, resolution 1.450Å" /> '''Crystal Structure o...
 
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[[Image:2f5t.gif|left|200px]]<br /><applet load="2f5t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2f5t, resolution 1.450&Aring;" />
'''Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB'''<br />


==Overview==
==Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB==
TrmB is an alpha-glucoside-sensing transcriptional regulator controlling, two operons encoding maltose/trehalose and maltodextrin ABC transporters, of Pyrococcus furiosus. The crystal structure of an N-terminal truncated, derivative of TrmB (amino acids 2-109 deleted; TrmB(delta2-109)) was, solved at 1.5 A resolution. This protein has lost its DNA binding domain, but has retained its sugar recognition site. The structure represents a, novel sugar-binding fold. TrmB(delta2-109) bound maltose, glucose, sucrose, and maltotriose, exhibiting Kd values of 6.8, 25, 34, and 160, microM, respectively. TrmB(delta2-109) behaved as a monomer in dilute, buffer solution in contrast to the full-length protein, which is a dimer., Co-crystallization with bound maltose identified a binding site involving, seven amino acid residues: Ser229, Asn305, Gly320, Met321, Val324, Ile325, and Glu326. Six of these residues interact with the nonreducing glucosyl, residue of maltose. The nonreducing glucosyl residue is shared by all, substrates bound to TrmB, suggesting it as a common recognition motif.
<StructureSection load='2f5t' size='340' side='right'caption='[[2f5t]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2f5t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5t OCA], [https://pdbe.org/2f5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5t RCSB], [https://www.ebi.ac.uk/pdbsum/2f5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRMBR_THELN TRMBR_THELN] Inhibits transcription of the trehalose/maltose transport gene cluster (malE operon). Acts by binding to two different operator sequences in the promoter, preventing polymerase recruitment and transcription.<ref>PMID:12426307</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f5t ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2F5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis] with <scene name='pdbligand=MAL:'>MAL</scene> and <scene name='pdbligand=IMD:'>IMD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5T OCA].
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of the sugar binding domain of the archaeal transcriptional regulator TrmB., Krug M, Lee SJ, Diederichs K, Boos W, Welte W, J Biol Chem. 2006 Apr 21;281(16):10976-82. Epub 2006 Feb 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16473881 16473881]
__TOC__
[[Category: Single protein]]
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermococcus litoralis]]
[[Category: Thermococcus litoralis]]
[[Category: Boos, W.]]
[[Category: Boos W]]
[[Category: Diederichs, K.]]
[[Category: Diederichs K]]
[[Category: Krug, M.]]
[[Category: Krug M]]
[[Category: Lee, S.J.]]
[[Category: Lee SJ]]
[[Category: Welte, W.]]
[[Category: Welte W]]
[[Category: IMD]]
[[Category: MAL]]
[[Category: sugar-binding]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:28:13 2008''

Latest revision as of 09:21, 14 February 2024

Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB

2f5t, resolution 1.45Å

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