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[[Image:1q3a.jpg|left|200px]]<br /><applet load="1q3a" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1q3a, resolution 2.10&Aring;" />
'''Crystal structure of the catalytic domain of human matrix metalloproteinase 10'''<br />


==Overview==
==Crystal structure of the catalytic domain of human matrix metalloproteinase 10==
The catalytic domain of matrix metalloproteinase-10 (MMP-10) has been, expressed in Escherichia coli and its crystal structure solved at 2.1 A, resolution. The availability of this structure allowed us to critically, examine the small differences existing between the catalytic domains of, MMP-3 and MMP-10, which show the highest sequence identity among all MMPs., Furthermore, the binding mode of, N-isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid (NNGH), which, is one of the most known commercial inhibitors of MMPs, is described for, the first time.
<StructureSection load='1q3a' size='340' side='right'caption='[[1q3a]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1q3a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q3A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NGH:N-ISOBUTYL-N-[4-METHOXYPHENYLSULFONYL]GLYCYL+HYDROXAMIC+ACID'>NGH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q3a OCA], [https://pdbe.org/1q3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q3a RCSB], [https://www.ebi.ac.uk/pdbsum/1q3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q3a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MMP10_HUMAN MMP10_HUMAN] Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q3/1q3a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q3a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The catalytic domain of matrix metalloproteinase-10 (MMP-10) has been expressed in Escherichia coli and its crystal structure solved at 2.1 A resolution. The availability of this structure allowed us to critically examine the small differences existing between the catalytic domains of MMP-3 and MMP-10, which show the highest sequence identity among all MMPs. Furthermore, the binding mode of N-isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid (NNGH), which is one of the most known commercial inhibitors of MMPs, is described for the first time.


==About this Structure==
Crystal structure of the catalytic domain of human matrix metalloproteinase 10.,Bertini I, Calderone V, Fragai M, Luchinat C, Mangani S, Terni B J Mol Biol. 2004 Feb 20;336(3):707-16. PMID:15095982<ref>PMID:15095982</ref>
1Q3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=NGH:'>NGH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Stromelysin_2 Stromelysin 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.22 3.4.24.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3A OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of the catalytic domain of human matrix metalloproteinase 10., Bertini I, Calderone V, Fragai M, Luchinat C, Mangani S, Terni B, J Mol Biol. 2004 Feb 20;336(3):707-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15095982 15095982]
</div>
<div class="pdbe-citations 1q3a" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Matrix metalloproteinase 3D structures|Matrix metalloproteinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Stromelysin 2]]
[[Category: Bertini I]]
[[Category: Bertini, I.]]
[[Category: Calderone V]]
[[Category: Calderone, V.]]
[[Category: Fragai M]]
[[Category: Fragai, M.]]
[[Category: Luchinat C]]
[[Category: Luchinat, C.]]
[[Category: Mangani S]]
[[Category: Mangani, S.]]
[[Category: Terni B]]
[[Category: Terni, B.]]
[[Category: CA]]
[[Category: NGH]]
[[Category: ZN]]
[[Category: hydroxamic acid]]
[[Category: inhibitors]]
[[Category: metalloproteinase]]
[[Category: mmp-10]]
[[Category: nngh]]
[[Category: stromelysin-2]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:42:32 2008''

Latest revision as of 09:56, 16 August 2023

Crystal structure of the catalytic domain of human matrix metalloproteinase 10

1q3a, resolution 2.10Å

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