4jax: Difference between revisions
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New page: '''Unreleased structure''' The entry 4jax is ON HOLD Authors: Kuettner, E.B., Strater, N., Kettner, K., Otto, A., Lilie, H., Golbik, R.P., Kriegel, T.M. Description: Crystal structure ... |
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The | ==Crystal structure of dimeric KlHxk1 in crystal form X== | ||
<StructureSection load='4jax' size='340' side='right'caption='[[4jax]], [[Resolution|resolution]] 2.26Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4jax]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JAX FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jax OCA], [https://pdbe.org/4jax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jax RCSB], [https://www.ebi.ac.uk/pdbsum/4jax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jax ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HXK_KLULA HXK_KLULA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The bifunctional hexokinase KlHxk1 is a key component of glucose dependent signal transduction in Kluyveromyces lactis. KlHxk1 is phosphorylated in vivo and undergoes ATP-dependent autophosphorylation-inactivation in vitro. This study identifies serine-15 as the site of in vivo phosphorylation and serine-157 as the autophosphorylation-inactivation site. X-ray crystallography of the in vivo phosphorylated enzyme indicates the existence of a ring-shaped symmetrical homodimer carrying two phosphoserine-15 residues. In contrast, small-angle X-ray scattering and equilibrium sedimentation analyses reveal the existence of monomeric phosphoserine-15 KlHxk1 in solution. While phosphorylation at serine-15 and concomitant homodimer dissociation are likely to be involved in glucose signalling, mechanism and putative physiological significance of KlHxk1 inactivation by autophosphorylation at serine-157 remain to be established. | |||
In vivo phosphorylation and in vitro autophosphorylation-inactivation of Kluyveromyces lactis hexokinase KlHxk1.,Kettner K, Kuettner EB, Otto A, Lilie H, Golbik RP, Strater N, Kriegel TM Biochem Biophys Res Commun. 2013 Apr 10. pii: S0006-291X(13)00600-1. doi:, 10.1016/j.bbrc.2013.03.121. PMID:23583397<ref>PMID:23583397</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4jax" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Hexokinase 3D structures|Hexokinase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Kluyveromyces lactis NRRL Y-1140]] | |||
[[Category: Large Structures]] | |||
[[Category: Golbik RP]] | |||
[[Category: Kettner K]] | |||
[[Category: Kriegel TM]] | |||
[[Category: Kuettner EB]] | |||
[[Category: Lilie H]] | |||
[[Category: Otto A]] | |||
[[Category: Strater N]] | |||
Latest revision as of 15:39, 20 September 2023
Crystal structure of dimeric KlHxk1 in crystal form X
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