4jbu: Difference between revisions
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New page: '''Unreleased structure''' The entry 4jbu is ON HOLD Authors: Lovell, Scott, Chaudhury, Sukanya, Battaile, Kevin P., Plano, Gregory, De Guzman, Roberto N. Description: 1.65A structure ... |
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The | ==1.65A structure of the T3SS tip protein LcrV (G28-D322, C273S) from Yersinia pestis== | ||
<StructureSection load='4jbu' size='340' side='right'caption='[[4jbu]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4jbu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JBU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jbu OCA], [https://pdbe.org/4jbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jbu RCSB], [https://www.ebi.ac.uk/pdbsum/4jbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jbu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LCRV_YERPE LCRV_YERPE] Possibly involved in calcium regulation of YOP expression, which includes the export process. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The human pathogen Yersinia pestis requires the assembly of the type III secretion system (T3SS) for virulence. The structural component of the T3SS contains an external needle and a tip complex, which is formed by LcrV in Y. pestis. The structure of an LcrV triple mutant (K40A/D41A/K42A) in a C273S background has previously been reported to 2.2 A resolution. Here, the crystal structure of LcrV without the triple mutation in a C273S background is reported at a higher resolution of 1.65 A. Overall the two structures are similar, but there are also notable differences, particularly near the site of the triple mutation. The refined structure revealed a slight shift in the backbone positions of residues Gly28-Asn43 and displayed electron density in the loop region consisting of residues Ile46-Val63, which was disordered in the original structure. In addition, the helical turn region spanning residues Tyr77-Gln95 adopts a different orientation. | |||
Structure of the Yersinia pestis tip protein LcrV refined to 1.65 A resolution.,Chaudhury S, Battaile KP, Lovell S, Plano GV, De Guzman RN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May;69(Pt 5):477-81. doi: , 10.1107/S1744309113008579. Epub 2013 Apr 27. PMID:23695558<ref>PMID:23695558</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4jbu" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Yersinia pestis]] | |||
[[Category: Battaile KP]] | |||
[[Category: Chaudhury S]] | |||
[[Category: De Guzman RN]] | |||
[[Category: Lovell S]] | |||
[[Category: Plano G]] | |||
Latest revision as of 15:39, 20 September 2023
1.65A structure of the T3SS tip protein LcrV (G28-D322, C273S) from Yersinia pestis
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