4hhm: Difference between revisions
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== | ==Crystal structure of a mutant, G219A, of Glucose Isomerase from Streptomyces sp. SK== | ||
[[4hhm]] is a 8 chain structure with sequence from [ | <StructureSection load='4hhm' size='340' side='right'caption='[[4hhm]], [[Resolution|resolution]] 2.15Å' scene=''> | ||
[[ | == Structural highlights == | ||
[[ | <table><tr><td colspan='2'>[[4hhm]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SK Streptomyces sp. SK]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HHM FirstGlance]. <br> | ||
[[Category: | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> | ||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hhm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hhm OCA], [https://pdbe.org/4hhm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hhm RCSB], [https://www.ebi.ac.uk/pdbsum/4hhm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hhm ProSAT]</span></td></tr> | ||
[[Category: | </table> | ||
[[Category: | == Function == | ||
[[Category: | [https://www.uniprot.org/uniprot/Q9ZAI3_9ACTN Q9ZAI3_9ACTN] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The role of residue 219 in the physicochemical properties of D-glucose isomerase from Streptomyces sp. SK strain (SKGI) was investigated by site-directed mutagenesis and structural studies. Mutants G219A, G219N, and G219F were generated and characterized. Comparative studies of their physicochemical properties with those of the wild-type enzyme highlighted that mutant G219A displayed increased specific activity and thermal stability compared to that of the wild-type enzyme, while for G219N and G219F, these properties were considerably decreased. A double mutant, SKGI F53L/G219A, displayed a higher optimal temperature and a higher catalytic efficiency than both the G219A mutant and the wild-type enzyme and showed a half-life time of about 150 min at 85 degrees C as compared to 50 min for wild-type SKGI. Crystal structures of SKGI wild-type and G219A enzymes were solved to 1.73 and 2.15 A, respectively, and showed that the polypeptide chain folds into two structural domains. The larger domain consists of a (beta/alpha)8 unit, and the smaller domain forms a loop of alpha helices. Detailed analyses of the three-dimensional structures highlighted minor but important changes in the active site region as compared to that of the wild-type enzyme leading to a displacement of both metal ions, and in particular that in site M2. The structural analyses moreover revealed how the substitution of G219 by an alanine plays a crucial role in improving the thermostability of the mutant enzyme. | |||
Identification of critical residues for the activity and thermostability of Streptomyces sp. SK glucose isomerase.,Ben Hlima H, Bejar S, Riguet J, Haser R, Aghajari N Appl Microbiol Biotechnol. 2013 Mar 6. PMID:23463249<ref>PMID:23463249</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4hhm" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[D-xylose isomerase 3D structures|D-xylose isomerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces sp. SK]] | |||
[[Category: Aghajari N]] | |||
[[Category: Ben Hlima H]] | |||
[[Category: Haser R]] | |||
[[Category: Riguet J]] | |||
Latest revision as of 15:06, 20 September 2023
Crystal structure of a mutant, G219A, of Glucose Isomerase from Streptomyces sp. SK
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