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{{STRUCTURE_2yn0|  PDB=2yn0  |  SCENE=  }}
===tau55 histidine phosphatase domain===
{{ABSTRACT_PUBMED_23569204}}


==Function==
==tau55 histidine phosphatase domain==
[[http://www.uniprot.org/uniprot/TFC7_YEAST TFC7_YEAST]] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes.<ref>PMID:9584160</ref>
<StructureSection load='2yn0' size='340' side='right'caption='[[2yn0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2yn0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YN0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yn0 OCA], [https://pdbe.org/2yn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yn0 RCSB], [https://www.ebi.ac.uk/pdbsum/2yn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yn0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TFC7_YEAST TFC7_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes.<ref>PMID:9584160</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Saccharomyces cerevisiae tau55, a subunit of the RNA polymerase III-specific general transcription factor TFIIIC, comprises an N-terminal histidine phosphatase domain (tau55-HPD) whose catalytic activity and cellular function is poorly understood. We solved the crystal structures of tau55-HPD and its closely related paralogue Huf and used in silico docking methods to identify phospho-serine and phospho-tyrosine containing peptides as possible substrates that were subsequently validated using in vitro phosphatase assays. A comparative phospho-proteomic study identified additional phosphopeptides as possible targets, which show the involvement of these two phosphatases in the regulation of a variety of cellular functions. Our results identify tau55-HPD and Huf as bona fide protein phosphatases, characterize their substrate specificities and provide a small set of regulated phosphosite targets in vivo.


==About this Structure==
Structural and functional characterization of a phosphatase domain within yeast general transcription factor TFIIIC.,Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grotsch H, Fernandez-Tornero C, Rybin V, Gavin AC, Kolb P, Muller CW J Biol Chem. 2013 Apr 8. PMID:23569204<ref>PMID:23569204</ref>
[[2yn0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN0 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<references group="xtra"/><references/>
</div>
[[Category: Saccharomyces cerevisiae s288c]]
<div class="pdbe-citations 2yn0" style="background-color:#fffaf0;"></div>
[[Category: Fernandez-Tornero, C.]]
 
[[Category: Gavin, A C.]]
==See Also==
[[Category: Glatt, S.]]
*[[Transcription factor tau|Transcription factor tau]]
[[Category: Grotsch, H.]]
== References ==
[[Category: Hennrich, M.]]
<references/>
[[Category: Kolb, P.]]
__TOC__
[[Category: Muller, C W.]]
</StructureSection>
[[Category: Rybin, V.]]
[[Category: Large Structures]]
[[Category: Scheven, G von.]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Taylor, N M.I.]]
[[Category: Fernandez-Tornero C]]
[[Category: Rna polymerase iii]]
[[Category: Gavin AC]]
[[Category: Transcription]]
[[Category: Glatt S]]
[[Category: Grotsch H]]
[[Category: Hennrich M]]
[[Category: Kolb P]]
[[Category: Muller CW]]
[[Category: Rybin V]]
[[Category: Taylor NMI]]
[[Category: Von Scheven G]]

Latest revision as of 13:37, 30 August 2023

tau55 histidine phosphatase domain

2yn0, resolution 1.50Å

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