2mme: Difference between revisions
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New page: '''Unreleased structure''' The entry 2mme is ON HOLD Authors: Demers, J., Habenstein, B.G., Loquet, A., Vasa, S., Becker, S., Baker, D., Lange, A., Sgourakis, N.G. Description: Hybrid ... |
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==Hybrid structure of the Shigella flexneri MxiH Type three secretion system needle== | |||
<SX load='2mme' size='340' side='right' viewer='molstar' caption='[[2mme]], [[Resolution|resolution]] 7.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mme]] is a 29 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MME FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , Solid-state NMR, [[Resolution|Resolution]] 7.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mme OCA], [https://pdbe.org/2mme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mme RCSB], [https://www.ebi.ac.uk/pdbsum/2mme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mme ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6XVY0_SHIFL Q6XVY0_SHIFL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state nuclear magnetic resonance (ssNMR) chemical shifts and distance constraints define the local secondary structure, protein fold and inter-subunit interactions. Finally, Rosetta structure calculations provide a general framework to integrate the different sources of structural information. Combining a 7.7-A cryo-EM density map and 996 ssNMR distance constraints, the structure of the type-III secretion system needle of Shigella flexneri is determined to a precision of 0.4 A. The calculated structures are cross-validated using an independent data set of 691 ssNMR constraints and scanning transmission electron microscopy measurements. The hybrid model resolves the conformation of the non-conserved N terminus, which occupies a protrusion in the cryo-EM density, and reveals conserved pore residues forming a continuous pattern of electrostatic interactions, thereby suggesting a mechanism for effector protein translocation. | |||
High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.,Demers JP, Habenstein B, Loquet A, Kumar Vasa S, Giller K, Becker S, Baker D, Lange A, Sgourakis NG Nat Commun. 2014 Sep 29;5:4976. doi: 10.1038/ncomms5976. PMID:25264107<ref>PMID:25264107</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2mme" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Shigella flexneri]] | |||
[[Category: Baker D]] | |||
[[Category: Becker S]] | |||
[[Category: Demers JP]] | |||
[[Category: Habenstein B]] | |||
[[Category: Lange A]] | |||
[[Category: Loquet A]] | |||
[[Category: Sgourakis NG]] | |||
[[Category: Vasa SK]] | |||
Latest revision as of 06:07, 15 May 2024
Hybrid structure of the Shigella flexneri MxiH Type three secretion system needle
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