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[[Image:1pjn.gif|left|200px]]


{{Structure
==Mouse Importin alpha-bipartite NLS N1N2 from Xenopus laevis phosphoprotein Complex==
|PDB= 1pjn |SIZE=350|CAPTION= <scene name='initialview01'>1pjn</scene>, resolution 2.50&Aring;
<StructureSection load='1pjn' size='340' side='right'caption='[[1pjn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1pjn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJN FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE= KPNA2 OR RCH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjn OCA], [https://pdbe.org/1pjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjn RCSB], [https://www.ebi.ac.uk/pdbsum/1pjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjn ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/HIBN_XENLA HIBN_XENLA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/1pjn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pjn ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Importin-alpha is the nuclear import receptor that recognizes cargo proteins carrying conventional basic monopartite and bipartite nuclear localization sequences (NLSs) and facilitates their transport into the nucleus. Bipartite NLSs contain two clusters of basic residues, connected by linkers of variable lengths. To determine the structural basis of the recognition of diverse bipartite NLSs by mammalian importin-alpha, we co-crystallized a non-autoinhibited mouse receptor protein with peptides corresponding to the NLSs from human retinoblastoma protein and Xenopus laevis phosphoprotein N1N2, containing diverse sequences and lengths of the linker. We show that the basic clusters interact analogously in both NLSs, but the linker sequences adopt different conformations, whereas both make specific contacts with the receptor. The available data allow us to draw general conclusions about the specificity of NLS binding by importin-alpha and facilitate an improved definition of the consensus sequence of a conventional basic/bipartite NLS (KRX10-12KRRK) that can be used to identify novel nuclear proteins.


'''Mouse Importin alpha-bipartite NLS N1N2 from Xenopus laevis phosphoprotein Complex'''
Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha.,Fontes MR, Teh T, Jans D, Brinkworth RI, Kobe B J Biol Chem. 2003 Jul 25;278(30):27981-7. Epub 2003 Apr 14. PMID:12695505<ref>PMID:12695505</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1pjn" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Importin-alpha is the nuclear import receptor that recognizes cargo proteins carrying conventional basic monopartite and bipartite nuclear localization sequences (NLSs) and facilitates their transport into the nucleus. Bipartite NLSs contain two clusters of basic residues, connected by linkers of variable lengths. To determine the structural basis of the recognition of diverse bipartite NLSs by mammalian importin-alpha, we co-crystallized a non-autoinhibited mouse receptor protein with peptides corresponding to the NLSs from human retinoblastoma protein and Xenopus laevis phosphoprotein N1N2, containing diverse sequences and lengths of the linker. We show that the basic clusters interact analogously in both NLSs, but the linker sequences adopt different conformations, whereas both make specific contacts with the receptor. The available data allow us to draw general conclusions about the specificity of NLS binding by importin-alpha and facilitate an improved definition of the consensus sequence of a conventional basic/bipartite NLS (KRX10-12KRRK) that can be used to identify novel nuclear proteins.
*[[Importin 3D structures|Importin 3D structures]]
 
== References ==
==About this Structure==
<references/>
1PJN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJN OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha., Fontes MR, Teh T, Jans D, Brinkworth RI, Kobe B, J Biol Chem. 2003 Jul 25;278(30):27981-7. Epub 2003 Apr 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12695505 12695505]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Brinkworth RI]]
[[Category: Brinkworth, R I.]]
[[Category: Fontes MRM]]
[[Category: Fontes, M R.M.]]
[[Category: Jans D]]
[[Category: Jans, D.]]
[[Category: Kobe B]]
[[Category: Kobe, B.]]
[[Category: Teh T]]
[[Category: Teh, T.]]
[[Category: bipartite nl]]
[[Category: importin alpha/karyopherin alpha]]
[[Category: nuclear localization sequence (nls) recognition]]
[[Category: xenopus laevis n1n2 phosphoprotein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:18 2008''

Latest revision as of 09:44, 16 August 2023

Mouse Importin alpha-bipartite NLS N1N2 from Xenopus laevis phosphoprotein Complex

1pjn, resolution 2.50Å

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