2fg5: Difference between revisions

Latest revision as of 09:23, 14 February 2024

Crystal structure of human RAB31 in complex with a GTP analogue

Structural highlights

2fg5 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.801Å
Ligands:	GNP, MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RAB31_HUMAN The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Ng EL, Wang Y, Tang BL. Rab22B's role in trans-Golgi network membrane dynamics. Biochem Biophys Res Commun. 2007 Sep 28;361(3):751-7. Epub 2007 Jul 30. PMID:17678623 doi:https://dx.doi.org/10.1016/j.bbrc.2007.07.076
↑ Lodhi IJ, Chiang SH, Chang L, Vollenweider D, Watson RT, Inoue M, Pessin JE, Saltiel AR. Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Cell Metab. 2007 Jan;5(1):59-72. PMID:17189207 doi:https://dx.doi.org/10.1016/j.cmet.2006.12.006
↑ Ng EL, Ng JJ, Liang F, Tang BL. Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells. J Cell Physiol. 2009 Dec;221(3):716-28. doi: 10.1002/jcp.21911. PMID:19725050 doi:https://dx.doi.org/10.1002/jcp.21911
↑ Kajiho H, Sakurai K, Minoda T, Yoshikawa M, Nakagawa S, Fukushima S, Kontani K, Katada T. Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31. J Biol Chem. 2011 Jul 8;286(27):24364-73. doi: 10.1074/jbc.M110.172445. Epub 2011, May 17. PMID:21586568 doi:10.1074/jbc.M110.172445
↑ Seto S, Tsujimura K, Koide Y. Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. Traffic. 2011 Apr;12(4):407-20. doi: 10.1111/j.1600-0854.2011.01165.x. Epub 2011 , Feb 21. PMID:21255211 doi:10.1111/j.1600-0854.2011.01165.x

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Ng EL, Wang Y, Tang BL. Rab22B's role in trans-Golgi network membrane dynamics. Biochem Biophys Res Commun. 2007 Sep 28;361(3):751-7. Epub 2007 Jul 30. PMID:17678623 doi:https://dx.doi.org/10.1016/j.bbrc.2007.07.076

[2] Lodhi IJ, Chiang SH, Chang L, Vollenweider D, Watson RT, Inoue M, Pessin JE, Saltiel AR. Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Cell Metab. 2007 Jan;5(1):59-72. PMID:17189207 doi:https://dx.doi.org/10.1016/j.cmet.2006.12.006

[3] Ng EL, Ng JJ, Liang F, Tang BL. Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells. J Cell Physiol. 2009 Dec;221(3):716-28. doi: 10.1002/jcp.21911. PMID:19725050 doi:https://dx.doi.org/10.1002/jcp.21911

[4] Kajiho H, Sakurai K, Minoda T, Yoshikawa M, Nakagawa S, Fukushima S, Kontani K, Katada T. Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31. J Biol Chem. 2011 Jul 8;286(27):24364-73. doi: 10.1074/jbc.M110.172445. Epub 2011, May 17. PMID:21586568 doi:10.1074/jbc.M110.172445

[5] Seto S, Tsujimura K, Koide Y. Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. Traffic. 2011 Apr;12(4):407-20. doi: 10.1111/j.1600-0854.2011.01165.x. Epub 2011 , Feb 21. PMID:21255211 doi:10.1111/j.1600-0854.2011.01165.x

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@@ Line 1: / Line 1: @@
-[[Image:2fg5.gif|left|200px]]
- {{Structure
+==Crystal structure of human RAB31 in complex with a GTP analogue==
-|PDB= 2fg5 |SIZE=350|CAPTION= <scene name='initialview01'>2fg5</scene>, resolution 2.801&Aring;
+<StructureSection load='2fg5' size='340' side='right'caption='[[2fg5]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene>
+<table><tr><td colspan='2'>[[2fg5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FG5 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.801&#8491;</td></tr>
-|GENE= RAB31, RAB22B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fg5 OCA], [https://pdbe.org/2fg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fg5 RCSB], [https://www.ebi.ac.uk/pdbsum/2fg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fg5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RAB31_HUMAN RAB31_HUMAN] The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.<ref>PMID:17678623</ref> <ref>PMID:17189207</ref> <ref>PMID:19725050</ref> <ref>PMID:21586568</ref> <ref>PMID:21255211</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/2fg5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fg5 ConSurf].
+<div style="clear:both"></div>
-'''Crystal structure of human RAB31 in complex with a GTP analogue'''
+==See Also==
+*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-FG5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FG5 OCA].
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Arrowsmith, C.]]
+[[Category: Arrowsmith C]]
-[[Category: Bochkarev, A.]]
+[[Category: Bochkarev A]]
-[[Category: Edwards, A.]]
+[[Category: Edwards A]]
-[[Category: Ismail, S.]]
+[[Category: Ismail S]]
-[[Category: Park, H.]]
+[[Category: Park H]]
-[[Category: SGC, Structural Genomics Consortium.]]
+[[Category: Sundstrom M]]
-[[Category: Sundstrom, M.]]
+[[Category: Tempel W]]
-[[Category: Tempel, W.]]
+[[Category: Wang J]]
-[[Category: Wang, J.]]
+[[Category: Weigelt J]]
-[[Category: Weigelt, J.]]
-[[Category: GNP]]
-[[Category: MG]]
-[[Category: g-protein]]
-[[Category: gtp analogue]]
-[[Category: rab]]
-[[Category: sgc]]
-[[Category: structural genomic]]
-[[Category: structural genomics consortium]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:02 2008''

2fg5: Difference between revisions

Latest revision as of 09:23, 14 February 2024

Crystal structure of human RAB31 in complex with a GTP analogue

Structural highlights

Function

Evolutionary Conservation

See Also

References

Contents

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