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| | <StructureSection load='' size='340' side='right' caption='Human cullin-1 (cyan) complex with ring box protein 1 (green) and Zn+2 ion (grey) (PDB code [[1ldj]])' scene='70/706699/Cv/2'> |
| | '''Cullins''' are hydrophobic scaffold proteins for E3 ubiquitin ligases. They combine with ring box proteins (RBX) to form the CRL complex.<ref>PMID:15688063</ref> The CRL complex is essential in targeting proteins to ubiquitin-mediated destruction. Cullin-1 to 7 are part of the multi-subunit ubiquitin complex. Conjugation of cullin to NEDD8 modulates the activation of the CRL.<br /> |
| | *'''Cullin-1''' is part of the SCF (Skp-cullin-F-box containing) complex which catalyzes the ubiquitination of proteins.<ref>PMID:11961546</ref><br /> |
| | *'''Cullin-2''' is part of the ECS (Elongin-cullin-SOCS-box) complex.<ref>PMID:25661653</ref><br /> |
| | *'''Cullin-3''' is part of the BTB-Cul3 complex.<ref>PMID:23349464</ref> <br /> |
| | *'''Cullin-4A and 4B''' regulate various processes like DNA repair, chromatin remodeling and mitotic cell cycle.<ref>PMID:22118460</ref><br /> |
| | *'''Cullin-5''' inhibits cellular proliferation.<ref>PMID:18805092</ref><br /> |
| | *'''Cullin-7''' is a core component of a complex which mediates ubiquitination of target proteins.<ref>PMID:17298945</ref><br /> |
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| <StructureSection load='1ldj' size='340' side='right' caption='Human cullin-1 complex with ring box protein 1 (PDB code [[1ldj]])' scene=''> | | <scene name='70/706699/Cv/7'>Human cullin-1 complex with ring box protein 1 and Zn+2 ions</scene>. |
| '''Cullins''' are hydrophobic scaffold proteins for E3 ubiquitin ligases. They combine with ring box proteins (RBX) to form the CRL complex. The CRL complex is essential in targeting proteins to ubiquitin-mediated destruction. Cullin-1 to 7 are part of the multi-subunit ubiquitin complex. Conjugation of cullin to NEDD8 modulates the activation of the CRL.<br />
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| *'''Cullin-1''' is part of the SCF (Skp-cullin-F-box containing) complex which catalyzes the ubiquitination of proteins.<br />
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| *'''Cullin-2''' is part of the ECS (Elongin-cullin-SOCS-box) complex.<br />
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| *'''Cullin-3''' is part of the BTB-Cul3 complex. <br />
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| *'''Cullin-4A and 4B''' regulate various processes like DNA repair, chromatin remodeling and mitotic cell cycle.<br />
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| *'''Cullin-5''' inhibits cellular proliferation.<br />
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| | <scene name='70/706699/Cv/8'>1st Zn coordination site</scene>. |
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| == Function == | | <scene name='70/706699/Cv/9'>2nd Zn coordination site</scene>. |
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| == Disease == | | <scene name='70/706699/Cv/10'>3rd Zn coordination site</scene>.(PDB code [[1ldj]]) |
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| == Relevance == | | == 3D Structures of cullin== |
| | | [[Cullin 3D structures]] |
| == Structural highlights ==
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| </StructureSection> | | </StructureSection> |
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| == 3D Structures of cullin==
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Cullin-1
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| **[[1ldj]] – hCul1 + RBX1 – human <br />
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| **[[3rtr]] – hCul1 residues 411-766 (mutant) + RBX1 <br />
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| **[[3tdu]] – hCul1 residues 702-776 + Dcn1-like protein + NEDD8-conjugating enzyme Ubc12 <br />
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| **[[3tdz]] – hCul1 residues 702-776 + Dcn1-like protein + stapled Ubc12 peptide <br />
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| **[[1ldk]] – hCul1 + RBX1 + cyclin A/Cdk2-asociated protein + Skp2-like protein <br />
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| **[[1u6g]] – hCul1 + TIP120 protein <br />
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| **[[4f52]] – hCul1 residues 411-690 (mutant) + RBX1 + glomulin<br />
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| **[[4p5o]] – hCul1 residues 411-776 + Dcn1-like protein + NEDD8-conjugating enzyme Ubc12 + RBX1 + NEDD8-conjugating enzyme Ubc12 + NEDD8 <br />
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| *Cullin-2
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| **[[4wqo]] – hCul2 residues 20-122 + PVHL + transcription elongation factor B <br />
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| *Cullin-3
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| **[[4ap2]], [[4apf]], [[4hxi]] – hCul3 N terminal (mutant) + kelch-like protein <br />
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| **[[4eoz]] – hCul3 N terminal (mutant) + peckle-type POZ protein <br />
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| **[[2myl]], [[2mym]] – hCul3 residues 49-68 - NMR <br />
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| *Cullin-4A
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| **[[2hye]] – hCul4A + RBX1 + DNA damage-binding protein 1 + nonstructural protein <br />
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| **[[4a0k]] – hCul4A + RBX1 + DNA damage-binding protein 1 + DNA damage-binding protein 2 + DNA<br />
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| *Cullin-4B
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| **[[4a64]] – hCul4B residues 188-539 <br />
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| **[[2do7]] – hCul4B winged helix-turn-helix - NMR <br />
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| **[[4a0c]] – hCul4B residues 192-913 + RBX1 + cullin-associated NEDD8-dissociated protein <br />
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| **[[4a0l]] – hCul4B + RBX1 + DNA damage-binding protein 1 + DNA damage-binding protein 2 + DNA<br />
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| *Cullin-5
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| **[[2wzk]] – hCul5 N terminal (mutant) <br />
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| **[[3dpl]] – hCul5 residues 401-780 (mutant) + RBX1 <br />
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| **[[3dqv]] – hCul5 residues 401-780 (mutant) + RBX1 + NEDD8 <br />
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| **[[4jgh]] – hCul5 N terminal (mutant) + SOCS-2 + transcription elongation factor B <br />
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| **[[4n9f]] – hCul5 N terminal + core-binding factor + virion infectivity factor + transcription elongation factor B <br />
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| *Cullin-7
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| **[[2jng]] – hCul7 residues 360-460 - NMR <br />
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| *Cullin-A
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| **[[3o2p]], [[3o6b]] – CulA residues 730-815 + defective in cullin neddylation protein - yeast
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| | [[Category:Topic Page]] |
