BtuB: Difference between revisions

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== Function ==

'''BtuB''' is an outer membrane receptor found in a variety of bacteria, such as ''E. coli''. BtuB transports vitamin B12 across the membrane of gram-negative bacteria. The transport is achieved with high affinity by the collaboration of BtuB and the periplasmic protein TonB. As an essential receptor for the cell that is constitutively expressed, it is an ideal target to be parasitized, a feature exploited by a variety of proteins such as [[Colicin]]s.

'''BtuB''' or '''vitamin B12 receptor''' or '''outer membrane cobalamin transporter''' is an outer membrane receptor found in a variety of bacteria, such as ''E. coli''. BtuB transports vitamin B12 across the membrane of gram-negative bacteria. The transport is achieved with high affinity by the collaboration of BtuB and the periplasmic protein TonB. <ref>PMID:14499604</ref> As an essential receptor for the cell that is constitutively expressed, it is an ideal target to be parasitized, a feature exploited by a variety of proteins such as [[Colicin]]s.

== Structural highlights ==

BtuB depends on the presence of Ca+2 ions for high affinity binding of cobalamin (a form of vitamin B12). The Ca+2 ions are coordinated to several Asp side chains.

BtuB depends on the presence of Ca+2 ions for high affinity <scene name='43/439262/Cv/6'>binding of cobalamin (a form of vitamin B12)</scene>. The <scene name='43/439262/Cv/7'>Ca+2 ions are coordinated to several Asp side chains</scene>.<ref>PMID:20816073</ref>

==3D structure of BtuB==

[[BtuB 3D structures]]

~~Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~

</StructureSection>

~~[[2guf]], [[1nqe]], [[1nqf]] – EcBtuB – ''Escherichia coli'' ~~

~~[[3m8b]], [[3rgm]], [[3rgn]] – EcBtuB (mutant) ~~

~~[[3m8d]] - EcBtuB (mutant) + cyanocobalamin ~~

~~[[1nqh]] - EcBtuB + cyanocobalamin + Ca ~~

~~[[2ysu]] - EcBtuB + Colicin E2 receptor binding domain ~~

~~[[1ujw]] - EcBtuB + Colicin E3 receptor binding domain ~~

~~[[2gsk]] - EcBtuB + TonB C-terminal ~~

~~[[1nqg]] - EcBtuB + Ca ~~

~~[[2bto]] – PdBtuBA + thioredoxin – ''Prosthecobacter dejongeii'' ~~

~~[[2btq]] – PdBtuBA + PdBtuBB~~

== References ==

[[Category:Topic Page]]

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@@ Line 3: / Line 3: @@
 == Function ==
-'''BtuB''' is an outer membrane receptor found in a variety of bacteria, such as ''E. coli''. BtuB transports vitamin B12 across the membrane of gram-negative bacteria.  The transport is achieved with high affinity by the collaboration of BtuB and the periplasmic protein TonB.  As an essential receptor for the cell that is constitutively expressed, it is an ideal target to be parasitized, a feature exploited by a variety of proteins such as [[Colicin]]s.
+'''BtuB''' or '''vitamin B12 receptor''' or '''outer membrane cobalamin transporter''' is an outer membrane receptor found in a variety of bacteria, such as ''E. coli''. BtuB transports vitamin B12 across the membrane of gram-negative bacteria.  The transport is achieved with high affinity by the collaboration of BtuB and the periplasmic protein TonB. <ref>PMID:14499604</ref> As an essential receptor for the cell that is constitutively expressed, it is an ideal target to be parasitized, a feature exploited by a variety of proteins such as [[Colicin]]s.
 == Structural highlights ==
-BtuB depends on the presence of Ca+2 ions for high affinity binding of cobalamin (a form of vitamin B12).  The Ca+2 ions are coordinated to several Asp side chains.
+BtuB depends on the presence of Ca+2 ions for high affinity <scene name='43/439262/Cv/6'>binding of cobalamin (a form of vitamin B12)</scene>.  The <scene name='43/439262/Cv/7'>Ca+2 ions are coordinated to several Asp side chains</scene>.<ref>PMID:20816073</ref>
 ==3D structure of BtuB==
+[[BtuB 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-[[2guf]], [[1nqe]], [[1nqf]] – EcBtuB – ''Escherichia coli''<br />
-[[3m8b]], [[3rgm]], [[3rgn]] – EcBtuB (mutant) <br />
-[[3m8d]] - EcBtuB (mutant) + cyanocobalamin<br />
-[[1nqh]] - EcBtuB + cyanocobalamin + Ca<br />
-[[2ysu]] - EcBtuB + Colicin E2 receptor binding domain<br />
-[[1ujw]] - EcBtuB + Colicin E3 receptor binding domain<br />
-[[2gsk]] - EcBtuB + TonB C-terminal<br />
-[[1nqg]] - EcBtuB + Ca<br />
-[[2bto]] – PdBtuBA + thioredoxin – ''Prosthecobacter dejongeii''<br />
-[[2btq]] – PdBtuBA + PdBtuBB
+== References ==
+<references/>
 [[Category:Topic Page]]