1aro: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1aro.jpg|left|200px]]


{{Structure
==T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME==
|PDB= 1aro |SIZE=350|CAPTION= <scene name='initialview01'>1aro</scene>, resolution 2.8&Aring;
<StructureSection load='1aro' size='340' side='right'caption='[[1aro]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>
<table><tr><td colspan='2'>[[1aro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ARO FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aro OCA], [https://pdbe.org/1aro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aro RCSB], [https://www.ebi.ac.uk/pdbsum/1aro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aro ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aro OCA], [http://www.ebi.ac.uk/pdbsum/1aro PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1aro RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/1aro_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aro ConSurf].
<div style="clear:both"></div>


'''T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME'''
==See Also==
 
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
==Overview==
__TOC__
The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression during infection of Escherichia coli. The 2.8 A crystal structure of the complex reveals that lysozyme binds at a site remote from the polymerase active site, suggesting an indirect mechanism of inhibition. Comparison of the T7 RNA polymerase structure with that of the homologous pol I family of DNA polymerases reveals identities in the catalytic site but also differences specific to RNA polymerase function. The structure of T7 RNA polymerase presented here differs significantly from a previously published structure. Sequence similarities between phage RNA polymerases and those from mitochondria and chloroplasts, when interpreted in the context of our revised model of T7 RNA polymerase, suggest a conserved fold.
</StructureSection>
 
[[Category: Escherichia phage T7]]
==About this Structure==
[[Category: Large Structures]]
1ARO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARO OCA].
[[Category: Jeruzalmi D]]
 
[[Category: Steitz T]]
==Reference==
Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme., Jeruzalmi D, Steitz TA, EMBO J. 1998 Jul 15;17(14):4101-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9670025 9670025]
[[Category: DNA-directed RNA polymerase]]
[[Category: Enterobacteria phage t7]]
[[Category: Protein complex]]
[[Category: Jeruzalmi, D.]]
[[Category: Steitz, T.]]
[[Category: complex (polymerase/hydrolase)]]
[[Category: dna-directed rna polymerase]]
[[Category: glycosidase]]
[[Category: hydrolase]]
[[Category: transcription]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:46:14 2008''

Latest revision as of 06:32, 7 February 2024

T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME

1aro, resolution 2.80Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1aro&oldid=4040319"