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==X-ray structure of PsaA from Yersinia pestis, in complex with galactose and AEBSF==
==X-ray structure of PsaA from Yersinia pestis, in complex with galactose and AEBSF==
<StructureSection load='4f8l' size='340' side='right' caption='[[4f8l]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='4f8l' size='340' side='right'caption='[[4f8l]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4f8l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F8L FirstGlance]. <br>
<table><tr><td colspan='2'>[[4f8l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F8L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=GAI:GUANIDINE'>GAI</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">psaA, y2882, YPO1303, YP_1289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=GAI:GUANIDINE'>GAI</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8l OCA], [http://pdbe.org/4f8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f8l RCSB], [http://www.ebi.ac.uk/pdbsum/4f8l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8l ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8l OCA], [https://pdbe.org/4f8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f8l RCSB], [https://www.ebi.ac.uk/pdbsum/4f8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8l ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PSAA_YERPE PSAA_YERPE]] Fibrillar structure, part of fimbriae, necessary for full virulence.  
[https://www.uniprot.org/uniprot/PSAA_YERPE PSAA_YERPE] Fibrillar structure, part of fimbriae, necessary for full virulence.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The pH 6 antigen (Psa) of Yersinia pestis consists of fimbriae that bind to two receptors: beta1-linked galactosyl residues in glycosphingolipids and the phosphocholine group in phospholipids. Despite the ubiquitous presence of either moiety on the surface of many mammalian cells, Y. pestis appears to prefer interacting with certain types of human cells, such as macrophages and alveolar epithelial cells of the lung. The molecular mechanism of this apparent selectivity is not clear. Site-directed mutagenesis of the consensus choline-binding motif in the sequence of PsaA, the subunit of the Psa fimbrial homopolymer, identified residues that abolish galactosylceramide binding, phosphatidylcholine binding, or both. The crystal structure of PsaA in complex with both galactose and phosphocholine reveals separate receptor binding sites that share a common structural motif, thus suggesting a potential interaction between the two sites. Mutagenesis of this shared structural motif identified Tyr126, which is part of the choline-binding consensus sequence but is found in direct contact with the galactose in the structure of PsaA, important for both receptor binding. Thus, this structure depicts a fimbrial subunit that forms a polymeric adhesin with a unique arrangement of dual receptor binding sites. These findings move the field forward by providing insights into unique types of multiple receptor-ligand interactions and should steer research into the synthesis of dual receptor inhibitor molecules to slow down the rapid progression of plague.
 
Structural basis for the specific recognition of dual receptors by the homopolymeric pH 6 antigen (Psa) fimbriae of Yersinia pestis.,Bao R, Nair MK, Tang WK, Esser L, Sadhukhan A, Holland RL, Xia D, Schifferli DM Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1065-70. doi:, 10.1073/pnas.1212431110. Epub 2012 Dec 31. PMID:23277582<ref>PMID:23277582</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4f8l" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Adhesin|Adhesin]]
*[[Adhesin 3D structures|Adhesin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bao, R]]
[[Category: Large Structures]]
[[Category: Esser, L]]
[[Category: Yersinia pestis]]
[[Category: Xia, D]]
[[Category: Bao R]]
[[Category: Adhesion]]
[[Category: Esser L]]
[[Category: All beta-strand]]
[[Category: Xia D]]
[[Category: Antigen]]
[[Category: Bacterial protein]]
[[Category: Cell adhesion-inhibitor complex]]
[[Category: Fimbriae]]
[[Category: Ig-fold]]
[[Category: Molecular sequence data]]
[[Category: Protein folding]]

Latest revision as of 11:11, 1 March 2024

X-ray structure of PsaA from Yersinia pestis, in complex with galactose and AEBSF

4f8l, resolution 1.50Å

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