5l2r: Difference between revisions
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New page: '''Unreleased structure''' The entry 5l2r is ON HOLD Authors: Feliciano, P.R., Drennan, C.L., Nonato, M.C. Description: Crystal structure of fumarate hydratase from Leishmania major [[... |
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==Crystal structure of fumarate hydratase from Leishmania major== | |||
<StructureSection load='5l2r' size='340' side='right'caption='[[5l2r]], [[Resolution|resolution]] 2.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5l2r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L2R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.054Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l2r OCA], [https://pdbe.org/5l2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l2r RCSB], [https://www.ebi.ac.uk/pdbsum/5l2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l2r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/FUM2_LEIMA FUM2_LEIMA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fumarate hydratases (FHs) are essential metabolic enzymes grouped into two classes. Here, we present the crystal structure of a class I FH, the cytosolic FH from Leishmania major, which reveals a previously undiscovered protein fold that coordinates a catalytically essential [4Fe-4S] cluster. Our 2.05 A resolution data further reveal a dimeric architecture for this FH that resembles a heart, with each lobe comprised of two domains that are arranged around the active site. Besides the active site, where the substrate S-malate is bound bidentate to the unique iron of the [4Fe-4S] cluster, other binding pockets are found near the dimeric enzyme interface, some of which are occupied by malonate, shown here to be a weak inhibitor of this enzyme. Taken together, these data provide a framework both for investigations of the class I FH catalytic mechanism and for drug design aimed at fighting neglected tropical diseases. | |||
Crystal structure of an Fe-S cluster-containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold.,Feliciano PR, Drennan CL, Nonato MC Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9804-9. doi:, 10.1073/pnas.1605031113. Epub 2016 Aug 15. PMID:27528683<ref>PMID:27528683</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5l2r" style="background-color:#fffaf0;"></div> | ||
[[Category: Drennan | == References == | ||
[[Category: Feliciano | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Leishmania major]] | |||
[[Category: Drennan CL]] | |||
[[Category: Feliciano PR]] | |||
[[Category: Nonato MC]] | |||
Latest revision as of 12:28, 13 August 2026
Crystal structure of fumarate hydratase from Leishmania major
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