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New page: ==Structure of human Rab8a in complex with the bMERB domain of Mical-cL== <StructureSection load='5szi' size='340' side='right' caption='5szi, resolution 2.85Å' s...
 
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==Structure of human Rab8a in complex with the bMERB domain of Mical-cL==
==Structure of human Rab8a in complex with the bMERB domain of Mical-cL==
<StructureSection load='5szi' size='340' side='right' caption='[[5szi]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
<StructureSection load='5szi' size='340' side='right'caption='[[5szi]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5szi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SZI FirstGlance]. <br>
<table><tr><td colspan='2'>[[5szi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SZI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5szi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5szi OCA], [http://pdbe.org/5szi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5szi RCSB], [http://www.ebi.ac.uk/pdbsum/5szi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5szi ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5szi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5szi OCA], [https://pdbe.org/5szi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5szi RCSB], [https://www.ebi.ac.uk/pdbsum/5szi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5szi ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref> [[http://www.uniprot.org/uniprot/MICLK_HUMAN MICLK_HUMAN]] May cooperate with MAPK1/ERK2 via an intracellular signal transduction pathway in the morphogenetic development of round spermatids to spermatozoa.
[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In their active GTP-bound form, Rab proteins interact with proteins termed effector molecules. In this study we have thoroughly characterised a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. Within our study, we show that these effectors display a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate binding sites. Structural analysis allowed us to explain the specificity towards Rab8 family members and the presence of two similar Rab binding sites that must have evolved via gene duplication. This study is the first to thoroughly characterise a Rab effector protein that contains two separate Rab binding sites within a single domain, allowing Micals and EHBPs to bind two Rabs simultaneously, thus suggesting previously unknown functions of these effector molecules in endosomal trafficking.


bMERB domains are bivalent Rab8 family effectors evolved by gene duplication.,Rai A, Oprisko A, Campos J, Fu Y, Friese T, Itzen A, Goody RS, Gazdag EM, Muller MP Elife. 2016 Aug 23;5. pii: e18675. doi: 10.7554/eLife.18675. PMID:27552051<ref>PMID:27552051</ref>
==See Also==
 
*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5szi" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Campos, J]]
[[Category: Homo sapiens]]
[[Category: Friese, T]]
[[Category: Large Structures]]
[[Category: Fu, Y]]
[[Category: Campos J]]
[[Category: Gazdag, E M]]
[[Category: Friese T]]
[[Category: Goody, R S]]
[[Category: Fu Y]]
[[Category: Itzen, A]]
[[Category: Gazdag EM]]
[[Category: Mueller, M P]]
[[Category: Goody RS]]
[[Category: Oprisko, A]]
[[Category: Itzen A]]
[[Category: Rai, A]]
[[Category: Mueller MP]]
[[Category: Duf3585]]
[[Category: Oprisko A]]
[[Category: Endocytosis]]
[[Category: Rai A]]
[[Category: Mical]]
[[Category: Mical-cl]]
[[Category: Rab effector]]
[[Category: Rab8a]]
[[Category: Transport protein]]

Latest revision as of 15:34, 6 March 2024

Structure of human Rab8a in complex with the bMERB domain of Mical-cL

5szi, resolution 2.85Å

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